FastQCFastQC Report
Wed 25 May 2016
SRR1780822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599844
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT100351.6729349630904036No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG27370.45628530084488633No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT16730.27890584885403535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9020.15037243016517626No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT8570.14287047965804442No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG150.002225088269.99441529
AGGGGGG17950.057.71608770
TGCGTAC200.006952618252.4958136
TATGCCG28450.050.6813743
CGTCTTC25700.050.66166348
CCGTCTT25450.050.60547
CGTATGC28750.050.39597341
GCCGTCT26100.050.2833446
CGTCAAC352.0862271E-550.00018348
AACTCGT1400.049.99601437
CTCGTAT29050.049.87553439
TCTTCTG24700.049.7453150
GTATGCC29200.049.7391842
TCGTATG29500.049.70789740
TCTCGTA29050.049.39364638
ATGCCGT28550.049.27802744
ATCTCGT29250.049.0559237
TGCCGTC28550.049.03286445
GCCGTCA3000.048.9960946
CTTGAAA28000.048.4961357
Produced by FastQC (version 0.11.5)