FastQCFastQC Report
Wed 25 May 2016
SRR1780821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199649
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3829419.180662061918667No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG3470.1738050278238308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC150.002220311770.015043
TCCACGA150.002222519669.997513
GACGACT150.002222519669.99756
TACGGAC308.344217E-658.34586338
TATATCG200.006937769752.51127643
ACCGACG200.00694464952.4981268
TGGTTAC200.00694464952.4981250
GCGTCGG200.00694464952.498128
TACCGAC200.00694464952.4981267
GCCGTAT552.2719178E-950.9072754
GTTCTAT305.8614585E-446.676691
TGGTCGC703.8198777E-1044.9983949
TCGCCGT757.512426E-1041.99849752
GATACCT459.17653E-538.88749728
CGGTGGT901.03682396E-1038.88749746
ATACCTC459.17653E-538.88749729
AAGATAC556.673081E-638.1804526
TCTCGGT852.5520421E-937.06678443
GTCGCCG852.557499E-937.057551
GTATCAT852.557499E-937.057557
Produced by FastQC (version 0.11.5)