FastQCFastQC Report
Wed 25 May 2016
SRR1780821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199649
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT177698.90011971009121No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG36011.8036654328346249No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT19450.9742097380903486No Hit
CTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT10400.5209142044287726No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT8370.4192357587566179No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT7610.3811689515098999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC7040.3526188460748614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6500.3255713777679828No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC4450.22289117401038824No Hit
TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG3990.19985073804526945No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCG3340.16729360026847118No Hit
CATAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT3230.16178393079855144No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT2830.14174876908975254RNA PCR Primer, Index 34 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2670.13373470440623292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGAC150.002221793470.0032618
ACGACTT252.83161E-670.0032647
ACGTAAT207.910775E-570.0032547
TCGGATC150.00222400369.9857233
CTCGAAA301.0213989E-769.9857239
ATCGGAT150.00222400369.9857232
TCGAAAG401.3460522E-1069.9857240
AGGGGGG27100.064.8532270
AAGCCGT1500.062.9871544
GACGACT401.0608346E-861.25284646
AGCCGTC1500.060.66948745
CGTAATC352.9573494E-760.01782648
CGACTTC900.058.35066248
TGCCGAC601.8189894E-1258.3360545
TAAGCCG1500.058.32143443
CGAATGC800.056.86339641
TGACGAC509.749783E-1056.0026145
GCCGACT750.056.00260546
ATGACGA509.767973E-1055.9885844
CCGACTT700.055.0025647
Produced by FastQC (version 0.11.5)