FastQCFastQC Report
Wed 25 May 2016
SRR1780820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176660
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72004.0756254953017095No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG3070.17378014264689234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT500.070.0314452
GCCGTAT500.070.0314454
CGTGATA252.8377854E-669.9719830
GTCGCCG600.064.1773151
TGGTCGC600.064.1773149
CGGTGGT600.064.1591446
TCGGTGG550.063.628945
TTCGTGA308.370731E-658.30998228
CTCGGTG653.6379788E-1253.85508344
GCGCTCT200.00693002152.52358654
GCTCAAT200.006937790752.5087136
GCGAAGT200.0069533552.47898532
CGTATCA707.2759576E-1250.0224656
CGTTGGC352.074714E-550.0224657
GCGTTGG352.074714E-550.0224656
TTTCGTG352.0851718E-549.97998427
ATGCTCG352.0851718E-549.97998424
ATCTCGG651.8371793E-1048.45585342
GATCTCG651.8371793E-1048.45585341
CGCCGTA751.4551915E-1146.68763453
Produced by FastQC (version 0.11.5)