FastQCFastQC Report
Wed 25 May 2016
SRR1780820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176660
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT7980.4517151590626062No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG7430.4205819087512736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5940.33623910336239105No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2800.1584965470395109No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC2230.12623117853503904No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT1950.11038152383108797TruSeq Adapter, Index 10 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG1820.1030227555756821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA252.8273043E-670.01556448
ACTTCGG150.00222231569.9957526
CGAATGC301.0197073E-769.9957541
ACGTATG150.00222231569.9957540
CGACTTC407.4365744E-752.51167748
CGTTGTT200.00694401652.49681546
TGACGAC200.00694401652.49681545
CTTGACG200.00694401652.49681527
GACGACT200.00694401652.49681546
TCGAATG352.0809866E-549.99696740
AAACTCG506.164555E-848.9970336
GTCTTCA751.4551915E-1146.6770549
TATGACG451.6783433E-646.66383743
CCTTGAG451.6783433E-646.66383747
AACTCGT305.8675813E-446.66383437
CAGCTTG851.8189894E-1245.30419554
CGTATGA404.5805948E-543.74734541
CACGTAT404.5805948E-543.74734539
AGCTTGA1000.041.9974555
AGGGGGG3700.040.68472370