FastQCFastQC Report
Wed 25 May 2016
SRR1780819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103832
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3057929.450458432853072No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG2930.28218660913783805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACATG150.002217185770.01591512
AGGGCAC150.002221431469.982173
AGCCGCA252.3781996E-456.0127364
CATGGAC200.00692807452.5119329
CGGCGTC200.00692807452.51193257
GCGCGCC200.00692807452.51193242
ATGGACA200.00692807452.51193210
CGTTCAA200.00692807452.51193241
ATTGGCG200.00692807452.51193237
CGCTGCG200.006941302752.48662616
GCACTGG200.006941302752.4866266
TGGTCGC751.4551915E-1146.67727349
TCGGTGG855.4569682E-1141.1858345
TCTCGGT701.8955689E-840.00909443
GCCGTAT701.8955689E-840.00909454
TGCTGGG350.00125206439.989812
CTCGGTG801.3951649E-939.3839544
CGGTGGT901.0186341E-1038.89772846
GTCGCCG753.4822733E-837.3418251
TATCATT852.5265763E-937.0672558
Produced by FastQC (version 0.11.5)