##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780819_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 103832 Sequences flagged as poor quality 0 Sequence length 76 %GC 64 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.121272825333232 32.0 27.0 32.0 13.0 32.0 2 28.86864357808768 32.0 32.0 32.0 13.0 32.0 3 28.712574158255645 32.0 27.0 32.0 13.0 32.0 4 28.112316049002235 32.0 27.0 32.0 13.0 32.0 5 28.526985900300485 32.0 27.0 32.0 13.0 32.0 6 32.49218930580168 37.0 32.0 37.0 13.0 37.0 7 32.18456737807227 37.0 32.0 37.0 13.0 37.0 8 32.37762924724555 37.0 32.0 37.0 13.0 37.0 9 32.50812851529393 37.0 32.0 37.0 13.0 37.0 10-11 32.17688188612374 37.0 32.0 37.0 13.0 37.0 12-13 32.04747091455428 37.0 32.0 37.0 13.0 37.0 14-15 32.72552777563757 37.0 32.0 37.0 13.0 37.0 16-17 32.68165401802912 37.0 32.0 37.0 13.0 37.0 18-19 32.193374874797755 37.0 32.0 37.0 13.0 37.0 20-21 32.56228811926959 37.0 32.0 37.0 13.0 37.0 22-23 32.505764118961395 37.0 32.0 37.0 13.0 37.0 24-25 32.14485900300485 37.0 32.0 37.0 13.0 37.0 26-27 32.53622197395793 37.0 32.0 37.0 13.0 37.0 28-29 32.38052816087526 37.0 32.0 37.0 13.0 37.0 30-31 32.28472436243162 37.0 32.0 37.0 13.0 37.0 32-33 32.434446991293626 37.0 32.0 37.0 13.0 37.0 34-35 32.29542433931736 37.0 32.0 37.0 13.0 37.0 36-37 32.8007502504045 37.0 32.0 37.0 13.0 37.0 38-39 32.81477771785191 37.0 32.0 37.0 13.0 37.0 40-41 32.36125664535018 37.0 32.0 37.0 13.0 37.0 42-43 32.24600315894907 37.0 32.0 37.0 13.0 37.0 44-45 31.987503852376918 37.0 32.0 37.0 13.0 37.0 46-47 32.180999113953305 37.0 32.0 37.0 13.0 37.0 48-49 32.6241669234918 37.0 32.0 37.0 13.0 37.0 50-51 32.7685154865552 37.0 32.0 37.0 13.0 37.0 52-53 31.948368518375837 37.0 32.0 37.0 13.0 37.0 54-55 32.04129266507435 37.0 32.0 37.0 13.0 37.0 56-57 31.849877687032897 37.0 32.0 37.0 13.0 37.0 58-59 31.579007435087448 37.0 32.0 37.0 13.0 37.0 60-61 31.36876396486632 37.0 29.5 37.0 13.0 37.0 62-63 31.130701517836506 37.0 27.0 37.0 13.0 37.0 64-65 31.139422336081363 37.0 29.5 37.0 13.0 37.0 66-67 31.18092206641498 37.0 27.0 37.0 13.0 37.0 68-69 31.299748632406196 37.0 29.5 37.0 13.0 37.0 70-71 31.43982587256337 37.0 32.0 37.0 13.0 37.0 72-73 31.669119346636876 37.0 32.0 37.0 13.0 37.0 74-75 31.95473457123045 37.0 32.0 37.0 13.0 37.0 76 31.89739194082749 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.7069598002232134 11101 2 -0.013180538599556257 11101 3 1.123665714138955 11101 4 0.19868635229088483 11101 5 -0.3814553232224718 11101 6 -0.11232967825956308 11101 7 1.2371765203794922 11101 8 0.9455728309794864 11101 9 -1.0376205995463756 11101 10-11 -0.6377609608738126 11101 12-13 0.3921067132775775 11101 14-15 0.7453693291349026 11101 16-17 -1.45077863865383 11101 18-19 0.40421218257630187 11101 20-21 -0.17531798639262774 11101 22-23 -1.5420012559905665 11101 24-25 -0.9453558818146277 11101 26-27 -1.4911378352838653 11101 28-29 1.2523051934824636 11101 30-31 1.070683580367593 11101 32-33 -0.08339997118715559 11101 34-35 0.0890080122249799 11101 36-37 1.0711228168065858 11101 38-39 -0.4324957549261015 11101 40-41 0.024079802178611942 11101 42-43 1.3482960787319627 11101 44-45 -1.1822147196749562 11101 46-47 0.8738397917149356 11101 48-49 0.38051796281965977 11101 50-51 0.6334187432464731 11101 52-53 -0.12814886689168858 11101 54-55 0.523695979555697 11101 56-57 -0.15999396566301272 11101 58-59 -0.2838687220459306 11101 60-61 -0.46243088092690954 11101 62-63 -0.798752133095519 11101 64-65 -1.3256805270270497 11101 66-67 -0.7042705895318164 11101 68-69 0.25220195415679925 11101 70-71 0.47485481221442427 11101 72-73 0.7972029753373207 11101 74-75 -0.3426954018844377 11101 76 -1.9408970445385734 11102 1 -1.167206866443454 11102 2 -0.08818053859955555 11102 3 -0.607167619194378 11102 4 -1.617146981042449 11102 5 -1.1289553232224705 11102 6 -4.148996344926228 11102 7 -1.780323479620506 11102 8 -0.7677605023538483 11102 9 -0.9584539328797064 11102 10-11 -1.8802609608738123 11102 12-13 -1.76747662005576 11102 14-15 -1.5571306708650994 11102 16-17 -2.48202863865383 11102 18-19 -0.9391211507570283 11102 20-21 -0.36281798639262774 11102 22-23 -1.4599179226572332 11102 24-25 -1.4924392151479609 11102 26-27 -1.3823878352838648 11102 28-29 -1.6785281398508651 11102 30-31 -0.5255664196324048 11102 32-33 -2.558399971187157 11102 34-35 -0.45390865444168327 11102 36-37 -2.576377183193415 11102 38-39 -1.4249957549261012 11102 40-41 -2.1055035311547172 11102 42-43 -1.055870587934706 11102 44-45 -4.015131386341622 11102 46-47 -1.656993541618398 11102 48-49 -1.3490653705136708 11102 50-51 -1.272831256753527 11102 52-53 -1.2598155335583527 11102 54-55 -1.9075540204443016 11102 56-57 -0.9291606323296762 11102 58-59 -2.6209520553792665 11102 60-61 -1.6982642142602415 11102 62-63 -1.0895854664288542 11102 64-65 -0.21568052702705032 11102 66-67 -2.6805205895318167 11102 68-69 -1.516548045843198 11102 70-71 -1.9784785211189124 11102 72-73 -1.5415470246626768 11102 74-75 -2.5847787352177676 11102 76 -1.7533970445385734 11103 1 1.8527931335565455 11103 2 1.1118194614004437 11103 3 -0.09614721103111279 11103 4 0.537546896508573 11103 5 0.8800242686142639 11103 6 0.6954934509921387 11103 7 0.22824794895092282 11103 8 0.42856602825839474 11103 9 -1.3747804634919518 11103 10-11 1.0650451615751635 11103 12-13 1.7188499105564858 11103 14-15 -0.04162046678347053 11103 16-17 -0.5585592508987247 11103 18-19 0.3514910941409326 11103 20-21 0.26473303401553494 11103 22-23 0.4896739140774642 11103 24-25 -0.3367249294336787 11103 26-27 0.0592448177773619 11103 28-29 0.24167594178178575 11103 30-31 0.5521886824084064 11103 32-33 0.40894696758835636 11103 34-35 -0.3053372258702538 11103 36-37 0.3581126127249519 11103 38-39 0.23459608180858993 11103 40-41 -0.3838708780934965 11103 42-43 0.1114763508408032 11103 44-45 1.4642563687604166 11103 46-47 1.0672921726673081 11103 48-49 0.5031795274455177 11103 50-51 -1.3136475832841406 11103 52-53 0.314878343992671 11103 54-55 -0.2014315714647097 11103 56-57 -0.756507571105189 11103 58-59 1.0492520262533844 11103 60-61 0.49887864288261596 11103 62-63 1.25694514581604 11103 64-65 0.17942151378927562 11103 66-67 0.6853977778151261 11103 68-69 -0.03287457645544478 11103 70-71 1.6007051523504714 11103 72-73 1.1243713426842632 11103 74-75 -0.8131460821565462 11103 76 1.2466029554614266 11104 1 -1.3614925807291698 11104 2 0.3439623185433014 11104 3 0.4956895236627652 11104 4 -1.0185755524710203 11104 5 0.3239018196346706 11104 6 0.5602893693594879 11104 7 0.10324794895092282 11104 8 0.6122394976461507 11104 9 1.3879746385488616 11104 10-11 -0.5574038180166667 11104 12-13 -0.04390519148432759 11104 14-15 -0.8974878137222397 11104 16-17 0.9554713613461701 11104 18-19 -1.4737640078998808 11104 20-21 -0.8628179863926277 11104 22-23 0.01901064877133507 11104 24-25 0.2972036419948978 11104 26-27 -0.30810212099815004 11104 28-29 -0.4024567112794344 11104 30-31 -0.5855664196324071 11104 32-33 0.1844571716699832 11104 34-35 -0.41248008301311145 11104 36-37 -1.1342343260505565 11104 38-39 0.08536138793103731 11104 40-41 -0.6351463882975743 11104 42-43 -1.2022991593632781 11104 44-45 -0.951559957770197 11104 46-47 -0.09342211304696946 11104 48-49 0.9534346294863312 11104 50-51 -0.7996169710392422 11104 52-53 -0.8573155335583529 11104 54-55 -0.7664825918728724 11104 56-57 -0.49630348947253466 11104 58-59 -1.496666341093551 11104 60-61 -2.1529070714030993 11104 62-63 -0.2660140378574276 11104 64-65 -0.5488948127413344 11104 66-67 -0.5837348752461011 11104 68-69 -1.7522623315574855 11104 70-71 -0.6709785211189114 11104 72-73 -0.8590470246626793 11104 74-75 -0.46620730664633925 11104 76 -1.6283970445385734 11105 1 1.203670326539001 11105 2 -0.4495840473714878 11105 3 -0.10330797007156889 11105 4 0.20636179088737805 11105 5 -1.4812360249768588 11105 6 -0.8096980993121932 11105 7 -1.1827796199713845 11105 8 -1.282497344459113 11105 9 -2.0847697223533928 11105 10-11 -1.165173241575566 11105 12-13 -1.264143286722426 11105 14-15 -1.799586811215974 11105 16-17 -1.8723795158468128 11105 18-19 -0.9685948349675542 11105 20-21 -0.06018640744525783 11105 22-23 -1.0425495016046042 11105 24-25 -0.745246232691823 11105 26-27 -1.4873001159856187 11105 28-29 -1.3299316486227966 11105 30-31 -0.13381203366749617 11105 32-33 -0.11629470802925823 11105 34-35 -0.7514525140908077 11105 36-37 -0.42778069196534574 11105 38-39 0.044477929284425954 11105 40-41 -0.9391877416810317 11105 42-43 -0.08043199144347568 11105 44-45 -0.505131386341624 11105 46-47 0.14248014259212027 11105 48-49 -0.10906537051366882 11105 50-51 -1.3342347655254585 11105 52-53 -0.07771027040045908 11105 54-55 -0.507554020444303 11105 56-57 -1.0207395796980983 11105 58-59 -0.39077661678277664 11105 60-61 -2.130194038821646 11105 62-63 -0.8327433611656971 11105 64-65 0.40677561332382695 11105 66-67 -0.3385907649704123 11105 68-69 -0.4621620809309199 11105 70-71 -0.542689047434699 11105 72-73 -0.7329505334346109 11105 74-75 -0.28951557732303357 11105 76 0.4571292712508992 11106 1 0.2867553977074877 11106 2 -0.699501293316537 11106 3 -0.5716959210811687 11106 4 -0.7631847168915051 11106 5 -1.0217855119017187 11106 6 0.338928183375657 11106 7 -1.804097064526168 11106 8 -1.7462510683915866 11106 9 -0.6905294045778199 11106 10-11 0.46011639761675127 11106 12-13 -1.3899294502444377 11106 14-15 0.2300391404556592 11106 16-17 -0.8263682612953396 11106 18-19 -1.0187437922664593 11106 20-21 0.14189899473944934 11106 22-23 0.1610254735691825 11106 24-25 -1.2733826113743802 11106 26-27 -0.26767085415178826 11106 28-29 -2.6741885172093554 11106 30-31 -1.58084943850033 11106 32-33 -1.9583999711871556 11106 34-35 -2.281078465762441 11106 36-37 -0.8003394473443635 11106 38-39 -0.5280146228506268 11106 40-41 -1.16361673870189 11106 42-43 -0.13322907850074728 11106 44-45 -2.873055914643512 11106 46-47 -1.797936937844817 11106 48-49 -0.7411408422117809 11106 50-51 -1.9049067284516426 11106 52-53 -2.0518910052564685 11106 54-55 -1.1867993034631716 11106 56-57 -0.304254971952318 11106 58-59 -1.1968011119830386 11106 60-61 -1.4809057236942031 11106 62-63 -1.7110949003911173 11106 64-65 -1.3681333572157293 11106 66-67 -1.0633507782110598 11106 68-69 -1.990132951503579 11106 70-71 0.037747893975428326 11106 72-73 -1.0111696661721155 11106 74-75 -2.5268542069158855 11106 76 0.5484897479142603 11107 1 0.9194598002232119 11107 2 0.8284861280671088 11107 3 1.4861657141389557 11107 4 0.10285301895755339 11107 5 0.19771134344419394 11107 6 1.704336988407107 11107 7 1.3996765203794936 11107 8 -1.2210938356871814 11107 9 1.0915460671202908 11107 10-11 0.3414057057928517 11107 12-13 0.6858567132775732 11107 14-15 0.5078693291349055 11107 16-17 0.33463802801283293 11107 18-19 0.26254551590963615 11107 20-21 0.2121820136073751 11107 22-23 0.6767487440094371 11107 24-25 0.1692274515187009 11107 26-27 0.060945498049463254 11107 28-29 0.7981385268157979 11107 30-31 1.2061002470342572 11107 32-33 0.09160002881284157 11107 34-35 0.5910913455583184 11107 36-37 -0.24971051652674703 11107 38-39 1.3383375784072271 11107 40-41 0.8344964688452805 11107 42-43 2.260796078731957 11107 44-45 -0.5301313863416226 11107 46-47 0.18633979171493564 11107 48-49 -0.4840653705136688 11107 50-51 0.11050207657981304 11107 52-53 -0.2698155335583543 11107 54-55 0.24244597955569702 11107 56-57 1.0775060343369915 11107 58-59 -0.6609520553792692 11107 60-61 -0.5415975475935753 11107 62-63 1.0637478669044782 11107 64-65 -0.20068052702704975 11107 66-67 -0.5855205895318178 11107 68-69 1.6584519541567992 11107 70-71 0.24985481221442285 11107 72-73 0.02011964200398708 11107 74-75 0.6885545981155587 11107 76 0.21326962212809164 11108 1 -0.924984644221233 11108 2 0.5721369217179024 11108 3 0.13061015858340141 11108 4 0.12031333641786901 11108 5 -0.6959394502065983 11108 6 -0.560742376672259 11108 7 0.10126382196679629 11108 8 -0.16553828013162786 11108 9 0.04472067029489324 11108 10-11 -0.5732768338896861 11108 12-13 1.2993487767696337 11108 14-15 0.349535995801574 11108 16-17 -0.6606000672252605 11108 18-19 -0.02039099202686856 11108 20-21 0.4665470929724478 11108 22-23 -0.30737824011755066 11108 24-25 -0.4557725484812991 11108 26-27 -0.09381640671243474 11108 28-29 -0.3637662350889599 11108 30-31 0.6723700883040991 11108 32-33 0.422552409765224 11108 34-35 0.4565675360345054 11108 36-37 -0.1600279768442121 11108 38-39 -1.371979881910228 11108 40-41 0.4233853577341691 11108 42-43 1.085399253335133 11108 44-45 -0.5210044022146363 11108 46-47 -1.242231636856495 11108 48-49 -0.8471606086089061 11108 50-51 -0.7093391932614637 11108 52-53 0.8182797045368879 11108 54-55 0.2464142335239501 11108 56-57 -0.19074793391698108 11108 58-59 -1.2307933252205387 11108 60-61 -0.17572453172055802 11108 62-63 -0.6092680061113924 11108 64-65 -1.0439344952810181 11108 66-67 -0.42798090699213276 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0.8280064583816014 11109 48-49 -0.9340653705136681 11109 50-51 -0.27283125675352693 11109 52-53 -0.49481553355835217 11109 54-55 0.1424459795556956 11109 56-57 -0.45582729899634344 11109 58-59 -1.2859520553792692 11109 60-61 -0.7082642142602431 11109 62-63 -0.3862521330955211 11109 64-65 -0.7340138603603812 11109 66-67 0.014479410468183573 11109 68-69 1.050118620823465 11109 70-71 -1.1501451877855757 11109 72-73 -0.38821369132934436 11109 74-75 -0.7947787352177684 11109 76 -1.1700637112052412 11110 1 -0.6845202992792743 11110 2 0.5148045360273095 11110 3 0.6065637240892059 11110 4 0.2058380935844184 11110 5 0.04298497528499112 11110 6 -0.17078738970234753 11110 7 0.7250496547078527 11110 8 1.6868663633177903 11110 9 0.4863221865232745 11110 10-11 -0.5659326026648586 11110 12-13 -0.11986467975724935 11110 14-15 0.4589887321199768 11110 16-17 -0.2021778923851727 11110 18-19 0.39550571491461994 11110 20-21 0.6856894762939376 11110 22-23 -0.13170896743335447 11110 24-25 -0.9582601106703521 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21611 48-49 0.3785889504739899 21611 50-51 0.7209959037403024 21611 52-53 0.7159869355774511 21611 54-55 0.5480015351112542 21611 56-57 1.0614566516209436 21611 58-59 0.5822578211639424 21611 60-61 0.2528468968508655 21611 62-63 0.6958466323365755 21611 64-65 0.8872824359359122 21611 66-67 0.30923249688793675 21611 68-69 -0.2906221199172734 21611 70-71 0.48720049122676556 21611 72-73 -0.3567322098478627 21611 74-75 -0.024408364847396058 21611 76 -0.04969334083487098 21612 1 0.06011020672727696 21612 2 -0.5833024898190686 21612 3 -1.049606643584621 21612 4 0.6321213116404785 21612 5 0.5773861401921607 21612 6 0.15393048434206236 21612 7 0.17772530086729432 21612 8 1.6244346195973733 21612 9 0.7464241159007798 21612 10-11 0.04364147815057606 21612 12-13 0.2683770384808284 21612 14-15 1.274332743769051 21612 16-17 0.27406892232177427 21612 18-19 0.21722031265760222 21612 20-21 0.7835234770220083 21612 22-23 0.9856918334403275 21612 24-25 0.10926810192520975 21612 26-27 1.276392652521011 21612 28-29 0.7802523479540113 21612 30-31 1.3290677267090558 21612 32-33 0.3769658824713815 21612 34-35 0.8636523211680682 21612 36-37 1.0909398899773137 21612 38-39 0.15744326946414589 21612 40-41 0.40644768835748124 21612 42-43 0.5855928266994397 21612 44-45 0.14730763804862335 21612 46-47 0.4627625559425752 21612 48-49 0.7689834099741333 21612 50-51 1.4222906944659854 21612 52-53 0.6594527591245729 21612 54-55 1.0107386624825239 21612 56-57 0.9766930262069096 21612 58-59 2.08782843242561 21612 60-61 -0.0716788484065809 21612 62-63 0.6499267286930959 21612 64-65 -0.08177808800266106 21612 66-67 1.334601361687696 21612 68-69 -0.08240170437978733 21612 70-71 0.5439605032713324 21612 72-73 1.480891999727568 21612 74-75 1.2210749233188167 21612 76 1.088066370095575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 9.0 12 34.0 13 71.0 14 134.0 15 256.0 16 381.0 17 541.0 18 656.0 19 860.0 20 1062.0 21 1379.0 22 1778.0 23 2088.0 24 2338.0 25 2683.0 26 2978.0 27 3332.0 28 3672.0 29 4032.0 30 4866.0 31 5595.0 32 7044.0 33 9402.0 34 13735.0 35 18040.0 36 16864.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 61.56928085061302 14.11042944785276 6.810104881971665 17.510184819562557 2 47.655738336479565 15.468852332704088 21.7889201371499 15.08648919366645 3 46.53407175535757 16.638574524440163 20.25908981459186 16.568263905610404 4 43.634367385462916 14.386840153711319 23.376448266895245 18.602344193930524 5 43.40447663443387 23.06988480949262 23.833647956235314 9.691990599838194 6 53.18694019069633 25.25763266878551 12.530097274390831 9.025329866127324 7 51.7128795831688 21.157457791988904 14.126802207433235 13.00286041740906 8 50.5706498184515 24.184957959722237 13.111943676622136 12.132448545204133 9 49.94028700760859 12.927862852740054 13.206202446306461 23.92564769334489 10-11 51.22196699364818 17.953644712193665 17.09549882739325 13.728889466764906 12-13 50.315661733900285 16.838181826937433 16.606070529088555 16.240085910073727 14-15 50.31205455175868 16.910178371922797 15.159205609276881 17.618561467041648 16-17 49.933784402012954 18.981002142977534 15.269557679805445 15.815655775204066 18-19 49.00172398848106 18.768841075229464 16.889308382051258 15.340126554238218 20-21 49.72791539854374 18.544130677659208 16.425723311630776 15.302230612166277 22-23 50.143988134221985 18.21425819624764 16.079959163231496 15.56179450629888 24-25 49.566595074593806 18.97543075633975 16.100029856783753 15.357944312282696 26-27 49.62053721913494 18.636052816596198 16.674210977453747 15.069198986815113 28-29 50.13122348442894 18.483971472736815 17.20929784600864 14.175507196825595 30-31 50.44904484669874 18.034026282968078 16.628865036140287 14.888063834192899 32-33 49.402388519695656 18.701723971877108 16.94019069633054 14.955696812096697 34-35 50.110038284654834 18.298620307721944 16.833208928270448 14.758132479352772 36-37 50.58438837868945 17.452673450419688 17.230186898335205 14.732751272555658 38-39 49.92728568539261 18.004256917490924 17.424468607641263 14.643988789475204 40-41 49.87334457019023 17.634962677582468 17.606067902720923 14.885624849506382 42-43 49.20494274239374 17.97233913453852 17.709888374153657 15.11282974891408 44-45 48.53244983362146 17.827784974405155 18.199548297930743 15.440216894042639 46-47 49.13701770269489 17.51295436595843 17.913143143335965 15.43688478801071 48-49 48.78116903755213 17.565420065684926 18.903678163133613 14.749732733629333 50-51 49.15148946826031 17.418543952075048 18.244421115487967 15.185545464176675 52-53 48.24637018131035 18.038573595627362 18.231682357756856 15.483373865305433 54-55 48.11640125011437 18.39921795618821 17.712040296444687 15.772340497252735 56-57 48.30052202723788 18.16934101284842 17.526920039296513 16.003216920617184 58-59 48.26701084051299 17.97425438364147 17.993999431727882 15.76473534411766 60-61 47.886659250591116 18.225728003390206 17.810620398060262 16.076992347958413 62-63 47.79202142004084 18.00477713140964 18.010074353738876 16.19312709481065 64-65 48.39419608674086 18.3026491310022 17.667454840527224 15.635699941729714 66-67 48.41993739465447 17.888273537202025 17.09511196725259 16.596677100890922 68-69 48.52804492195965 18.427731412142606 16.733526287148024 16.310697378749715 70-71 49.29230858139053 18.24924029723521 16.519381450249703 15.939069671124553 72-73 49.857457935644184 17.9405561066754 15.956043109343247 16.24594284833717 74-75 50.75627338524436 17.543327410275303 15.867535381844625 15.832863822635712 76 51.86601304042146 16.32749371574963 15.818975065250262 15.987518178578652 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 1.5 8 0.0 9 1.5 10 5.0 11 7.0 12 7.0 13 9.5 14 22.0 15 24.0 16 21.5 17 27.0 18 31.0 19 43.0 20 62.0 21 73.0 22 107.5 23 155.0 24 205.5 25 243.0 26 323.0 27 413.0 28 489.0 29 555.0 30 677.5 31 874.0 32 1107.5 33 1267.0 34 1416.0 35 1743.5 36 2078.0 37 2234.0 38 2445.0 39 2909.5 40 3430.0 41 3778.5 42 3860.0 43 3979.0 44 4166.5 45 4293.0 46 4351.0 47 4362.0 48 4183.0 49 3758.5 50 3524.0 51 3256.5 52 2792.5 53 2419.5 54 2243.0 55 2075.5 56 1738.0 57 1421.0 58 1274.0 59 1056.5 60 784.0 61 666.5 62 604.0 63 580.5 64 513.0 65 412.5 66 340.5 67 325.0 68 289.5 69 234.5 70 204.5 71 194.0 72 190.0 73 189.5 74 179.0 75 165.0 76 145.0 77 123.5 78 118.0 79 114.0 80 117.0 81 123.0 82 124.0 83 122.0 84 123.0 85 123.5 86 135.5 87 148.0 88 153.5 89 173.0 90 207.5 91 240.5 92 253.0 93 273.0 94 347.5 95 484.5 96 567.0 97 736.5 98 1348.0 99 16581.5 100 31373.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.630942291393791E-4 2 0.0038523769165575163 3 0.006741659603975652 4 9.630942291393791E-4 5 0.0038523769165575163 6 0.0019261884582787582 7 9.630942291393791E-4 8 0.002889282687418137 9 0.0019261884582787582 10-11 0.003370829801987826 12-13 0.0024077355728484473 14-15 0.0038523769165575163 16-17 0.004333924031127205 18-19 0.002889282687418137 20-21 0.0038523769165575163 22-23 0.0038523769165575163 24-25 0.002889282687418137 26-27 9.630942291393791E-4 28-29 0.0014446413437090686 30-31 4.8154711456968954E-4 32-33 0.0019261884582787582 34-35 0.004333924031127205 36-37 0.005297018260266585 38-39 9.630942291393791E-4 40-41 0.006741659603975652 42-43 0.002889282687418137 44-45 0.0024077355728484473 46-47 0.005778565374836274 48-49 0.002889282687418137 50-51 0.002889282687418137 52-53 0.004333924031127205 54-55 0.0014446413437090686 56-57 0.005778565374836274 58-59 0.008186300947684721 60-61 0.003370829801987826 62-63 0.0038523769165575163 64-65 0.005297018260266585 66-67 0.006741659603975652 68-69 0.007223206718545343 70-71 0.008186300947684721 72-73 0.002889282687418137 74-75 4.8154711456968954E-4 76 9.630942291393791E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 103832.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.095307804915635 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61758691206545 37.9671007011326 2 9.100204498977506 8.571538639340472 3 4.492842535787321 6.347754064257646 4 2.296523517382413 4.32621927729409 5 1.2208588957055213 2.874836273981046 6 0.6584867075664622 1.8606980506972803 7 0.4396728016359919 1.4494568148547655 8 0.2556237218813906 0.9630942291393789 9 0.20040899795501022 0.8494491101009323 >10 0.7075664621676891 4.887703212882348 >50 0.006134969325153374 0.16950458432853072 >100 0.0020449897750511245 0.28218660913783805 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0020449897750511245 29.450458432853072 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 30579 29.450458432853072 No Hit CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG 293 0.28218660913783805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.07223206718545343 0.0 2 0.0 0.0 0.0 0.07608444410201094 0.0 3 0.0 0.0 0.0 0.07608444410201094 0.0 4 0.0 0.0 0.0 0.08378919793512597 0.0 5 0.0 0.0 0.0 0.08571538639340473 0.0 6 0.0 0.0 0.0 0.08571538639340473 0.0 7 0.0 0.0 0.0 0.08571538639340473 0.0 8 0.0 0.0 0.0 0.08571538639340473 0.0 9 0.0 0.0 0.0 0.08860466908082287 0.0 10 0.0 0.0 0.0 0.09053085753910163 0.0 11 0.0 0.0 0.0 0.09053085753910163 0.0 12 0.0 0.0 0.0 0.09245704599738039 0.0 13 0.0 0.0 0.0 0.09342014022651976 0.0 14 0.0 0.0 0.0 0.09823561137221666 0.0 15 0.0 0.0 0.0 0.10016179983049542 0.0 16 0.0 0.0 0.0 0.10016179983049542 0.0 17 0.0 0.0 0.0 0.10016179983049542 0.0 18 0.0 0.0 0.0 0.10112489405963479 0.0 19 0.0 0.0 0.0 0.1097927421218892 0.0 20 0.0 0.0 0.0 0.11557130749672548 0.0 21 0.0 0.0 0.0 0.11557130749672548 0.0 22 0.0 0.0 0.0 0.14061175745434934 0.0 23 0.0 0.0 0.0 0.147353417058325 0.0 24 0.0 0.0 0.0 0.14927960551660374 0.0 25 0.0 0.0 0.0 0.15409507666230063 0.0 26 0.0 0.0 0.0 0.15794745357885814 0.0 27 0.0 0.0 0.0 0.16372601895369443 0.0 28 0.0 0.0 0.0 0.1743200554742276 0.0 29 0.0 0.0 0.0 0.19839741120271207 0.0 30 0.0 0.0 0.0 0.2244009553894753 0.0 31 0.0 0.0 0.0 0.2484783111179598 0.0 32 0.0 0.0 0.0 0.3033746821789044 0.0 33 0.0 0.0 0.0 0.3351567917405039 0.0 34 0.0 0.0 0.0 0.38138531473919407 0.0 35 0.0 0.0 0.0 0.4940673395485014 0.0 36 0.0 0.0 0.0 0.6809076200015409 0.0 37 0.0 0.0 0.0 0.9053085753910163 0.0 38 0.0 0.0 0.0 1.1903844672162724 0.0 39 0.0 0.0 0.0 1.5342091070190307 0.0 40 0.0 0.0 0.0 1.9714538870483087 0.0 41 0.0 0.0 0.0 2.446259342014023 0.0 42 0.0 0.0 0.0 2.911433854688343 0.0 43 0.0 0.0 0.0 3.3785345558209414 0.0 44 0.0 0.0 0.0 3.898605439556206 0.0 45 0.0 0.0 0.0 4.453347715540488 0.0 46 0.0 0.0 0.0 4.980160258879729 0.0 47 0.0 0.0 0.0 5.4501502426997455 0.0 48 0.0 0.0 0.0 5.9307342630402955 0.0 49 0.0 0.0 0.0 6.3564219123199015 0.0 50 0.0 0.0 0.0 6.790777409661762 0.0 51 0.0 0.0 0.0 7.214538870483088 0.0 52 0.0 0.0 0.0 7.705716927344171 0.0 53 0.0 0.0 0.0 8.152592649664843 0.0 54 0.0 0.0 0.0 8.602357654672932 0.0 55 0.0 0.0 0.0 9.071384544263811 0.0 56 0.0 0.0 0.0 9.513444795438785 0.0 57 0.0 0.0 0.0 9.94105863317667 0.0 58 0.0 0.0 0.0 10.289698744125126 0.0 59 0.0 0.0 0.0 10.696124508821944 0.0 60 0.0 0.0 0.0 11.038986054395561 0.0 61 0.0 0.0 0.0 11.455042761383774 0.0 62 0.0 0.0 0.0 11.888435164496494 0.0 63 0.0 0.0 0.0 12.320864473380075 0.0 64 0.0 0.0 0.0 12.690692657369597 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACATG 15 0.0022171857 70.015915 12 AGGGCAC 15 0.0022214314 69.98217 3 AGCCGCA 25 2.3781996E-4 56.01273 64 CATGGAC 20 0.006928074 52.511932 9 CGGCGTC 20 0.006928074 52.511932 57 GCGCGCC 20 0.006928074 52.511932 42 ATGGACA 20 0.006928074 52.511932 10 CGTTCAA 20 0.006928074 52.511932 41 ATTGGCG 20 0.006928074 52.511932 37 CGCTGCG 20 0.0069413027 52.486626 16 GCACTGG 20 0.0069413027 52.486626 6 TGGTCGC 75 1.4551915E-11 46.677273 49 TCGGTGG 85 5.4569682E-11 41.18583 45 TCTCGGT 70 1.8955689E-8 40.009094 43 GCCGTAT 70 1.8955689E-8 40.009094 54 TGCTGGG 35 0.001252064 39.98981 2 CTCGGTG 80 1.3951649E-9 39.38395 44 CGGTGGT 90 1.0186341E-10 38.897728 46 GTCGCCG 75 3.4822733E-8 37.34182 51 TATCATT 85 2.5265763E-9 37.06725 58 >>END_MODULE