FastQCFastQC Report
Wed 25 May 2016
SRR1780819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103832
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1263812.171584867863473No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG29922.881577933585022No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT15781.51976269358194No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT7200.6934278449803529No Hit
CTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT6320.6086755528160875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6180.5951922336081362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC5960.5740041605670699No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT5560.5354803914014947No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3440.3313044148239464No Hit
TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3190.30722705909546194No Hit
CATAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2940.2831497033669774No Hit
TCTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCG2440.23499499191000847No Hit
CAAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2170.20899144772324524No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT1960.1887664689113183RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT1440.13868556899607057No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT1420.13675938053779182No Hit
CATATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1260.12134987287156175No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT1210.11653440172586485No Hit
TCTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1070.10305108251791356No Hit
CTAAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1060.10208798828877418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT207.889611E-570.00915526
CAACACG150.002218037470.00915536
ATATCGA207.889611E-570.00915537
GCCGACA252.820614E-670.00915546
CTCATTA150.002218037470.00915563
GATTCCC150.002218037470.00915530
TTACCAA207.889611E-570.00915532
CCCGGAT150.002218037470.00915527
AGACCGA207.908478E-569.9754325
AGGGGGG19300.065.4748970
CGGTTAC800.061.2580129
AACTCGT750.060.67460337
TCTTATG352.95664E-759.97894750
GTTACCA900.058.34096531
CAACTCG900.058.34096536
TGCCGAC1050.056.6740845
CACGTAT1000.056.00732439
CGTCTAC509.713403E-1055.98034348
GTCAACT252.3850157E-455.98034349
GGATCCC1400.055.00719530
Produced by FastQC (version 0.11.5)