FastQCFastQC Report
Wed 25 May 2016
SRR1780818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113260
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79186.990994172699983No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG2140.18894578845135088No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT150.002219714770.000011
GCGAACT150.002219714770.0000128
TCAGATG207.8978424E-570.000014
GCAGGCT150.002219714770.0000150
GCCGTAT553.45608E-1157.27272454
TCGCCGT452.674642E-854.44444752
CAGCATT200.006935945752.5000041
CAACACG200.006935945752.50000416
TGGTCGC407.4240415E-752.50000449
GTCGCCG506.1352694E-848.99999651
GTGGTCG602.5722147E-740.83333648
CGTATCA701.9021172E-840.056
GTCAGAT350.001250982340.03
CGCCGTA701.9021172E-840.053
CGGTGGT556.6459033E-638.18181646
TCTCGGT654.8185757E-737.69230743
GTATCAT654.8185757E-737.69230757
GATCTCG654.8185757E-737.69230741
TAGATCT753.4940967E-837.33333239
GCGTGTA852.5356712E-937.05882334
Produced by FastQC (version 0.11.5)