FastQCFastQC Report
Wed 25 May 2016
SRR1780818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113260
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5240.46265230443227967No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT3510.30990641003001945No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG1230.10859968214727177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA301.01596015E-770.0000131
CGTAAGA150.002219714770.0000141
TCGAGGC150.002219714770.0000131
TCGACTA150.002219714770.0000139
ATCGACT150.002219714770.0000138
TGCCGAA150.002219714770.0000145
CGACACT308.331615E-658.33333623
TACGCGG308.331615E-658.33333629
GCCGAAA252.3818132E-455.99999646
ATGACGA252.3818132E-455.99999644
CTATGCA200.006935945752.50000434
GCGCCTT200.006935945752.50000430
TTCGTTC200.006935945752.50000440
CGTCTTA407.4240415E-752.50000448
AGAACGG200.006935945752.50000427
GTGTGTC200.006935945752.50000436
AATCTCG200.006935945752.50000436
AACTCGT200.006935945752.50000437
GAATTAC200.006935945752.50000442
CCGTCTT750.051.33333247
Produced by FastQC (version 0.11.5)