##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113260 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.969618576726116 27.0 27.0 32.0 13.0 32.0 2 26.564170934133852 32.0 27.0 32.0 13.0 32.0 3 27.20361116016246 32.0 27.0 32.0 13.0 32.0 4 28.99479074695391 32.0 27.0 32.0 22.0 32.0 5 26.648295956206958 32.0 27.0 32.0 13.0 32.0 6 27.92811230796398 32.0 22.0 37.0 13.0 37.0 7 30.706763199717464 37.0 27.0 37.0 13.0 37.0 8 29.649770439696272 37.0 27.0 37.0 13.0 37.0 9 31.80392018364824 37.0 32.0 37.0 13.0 37.0 10-11 30.99017305315204 37.0 27.0 37.0 13.0 37.0 12-13 31.335577432456297 37.0 29.5 37.0 13.0 37.0 14-15 31.860392018364823 37.0 32.0 37.0 13.0 37.0 16-17 31.01565424686562 37.0 27.0 37.0 13.0 37.0 18-19 31.169031432103125 37.0 27.0 37.0 13.0 37.0 20-21 31.65955324033198 37.0 29.5 37.0 13.0 37.0 22-23 31.443766554829594 37.0 27.0 37.0 13.0 37.0 24-25 31.310392018364823 37.0 27.0 37.0 13.0 37.0 26-27 31.32899081758785 37.0 29.5 37.0 13.0 37.0 28-29 31.342954264524103 37.0 27.0 37.0 13.0 37.0 30-31 31.133634999117078 37.0 27.0 37.0 13.0 37.0 32-33 30.845024721878865 37.0 27.0 37.0 13.0 37.0 34-35 30.649474660074166 37.0 27.0 37.0 13.0 37.0 36-37 30.961345576549533 37.0 27.0 37.0 13.0 37.0 38-39 30.00534169168285 37.0 27.0 37.0 13.0 37.0 40-41 29.313089351933606 37.0 24.5 37.0 13.0 37.0 42-43 29.575591559244216 37.0 24.5 37.0 10.5 37.0 44-45 29.93904291011831 37.0 27.0 37.0 13.0 37.0 46-47 29.23712254988522 37.0 22.0 37.0 10.5 37.0 48-49 29.65913385131556 37.0 24.5 37.0 10.5 37.0 50-51 28.99325004414621 37.0 22.0 37.0 13.0 37.0 52-53 29.451968921066573 37.0 22.0 37.0 13.0 37.0 54-55 29.401324386367648 37.0 22.0 37.0 13.0 37.0 56-57 28.718020483842487 37.0 22.0 37.0 13.0 37.0 58-59 29.171494790746955 37.0 22.0 37.0 13.0 37.0 60-61 28.830240155394666 37.0 22.0 37.0 10.5 37.0 62-63 29.45033551121314 37.0 22.0 37.0 13.0 37.0 64-65 29.57814762493378 37.0 22.0 37.0 13.0 37.0 66-67 29.448101712873033 37.0 22.0 37.0 13.0 37.0 68-69 29.01422390958856 37.0 22.0 37.0 13.0 37.0 70-71 28.03890605686032 32.0 17.5 37.0 10.5 37.0 72-73 27.974258343634116 34.5 13.0 37.0 8.0 37.0 74-75 28.462749426099244 37.0 22.0 37.0 8.0 37.0 76 28.26697863323327 37.0 22.0 37.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -1.899977657178166 11101 2 -1.6342071265555056 11101 3 -3.675951355566326 11101 4 -0.2022936119921006 11101 5 -1.6935254743520716 11101 6 1.585726943878985 11101 7 -4.125525591381631 11101 8 -1.8825131552349568 11101 9 -1.0506420209431973 11101 10-11 -1.6004494220093015 11101 12-13 1.9627634554858062 11101 14-15 -0.4278326453011445 11101 16-17 -2.047122023290239 11101 18-19 -0.23261358453384418 11101 20-21 -1.8227206412108323 11101 22-23 -1.271235859851739 11101 24-25 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1.0380711850982358 21612 22-23 1.3864908323577119 21612 24-25 0.14202563605615381 21612 26-27 1.2308596539154273 21612 28-29 1.4320406046940164 21612 30-31 1.1378977328492432 21612 32-33 2.1221984800484783 21612 34-35 0.8392928941687856 21612 36-37 1.1549294269270547 21612 38-39 1.5214288988173514 21612 40-41 1.284221335676225 21612 42-43 1.9540080719692767 21612 44-45 0.7720928777566911 21612 46-47 0.3172400424559356 21612 48-49 1.4462213350065447 21612 50-51 1.625302613545518 21612 52-53 1.4711868801591166 21612 54-55 0.9433518946915775 21612 56-57 0.8359569343963003 21612 58-59 1.230060418350316 21612 60-61 2.0056022173201633 21612 62-63 0.964662853829708 21612 64-65 0.9945348294669003 21612 66-67 0.9774026732472869 21612 68-69 0.7390541786500435 21612 70-71 0.8053356661518514 21612 72-73 0.6531543562965716 21612 74-75 0.6601821621202788 21612 76 1.373424518127294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 6.0 17 22.0 18 64.0 19 247.0 20 572.0 21 1337.0 22 2372.0 23 3370.0 24 4538.0 25 5832.0 26 7142.0 27 8191.0 28 8943.0 29 9837.0 30 11073.0 31 12281.0 32 12816.0 33 12708.0 34 9013.0 35 2748.0 36 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.83576448981034 20.997421679087346 12.292939639035072 16.873874192067248 2 28.78623332362108 30.007328335937984 14.525115001898303 26.681323338542633 3 22.03072576372947 32.08546706692566 20.231326152216138 25.652481017128732 4 16.22549885219848 24.347518982871268 33.154688327741475 26.27229383718877 5 15.782270881158396 39.36429454352817 21.870033551121317 22.983401024192123 6 24.881683972593063 41.88299074662711 18.61534929716748 14.619975983612346 7 27.167818892979806 34.03967896590999 18.953019186113245 19.839482954996953 8 24.241568073459298 38.944905527105774 16.869150626876213 19.944375772558715 9 24.65124492318559 25.029136500088295 17.61698746247572 32.702631114250394 10-11 24.179321914179763 29.370916475366414 24.6150450291365 21.834716581317323 12-13 24.658520003178612 24.776834985916985 25.72556221690492 24.839082793999488 14-15 23.10524457001589 24.795161575136852 21.57116369415504 30.52843016069221 16-17 26.012175686593057 29.34746619459025 21.272575568279645 23.367782550537044 18-19 25.537700865265762 28.325975631290834 22.994437577255873 23.141885926187534 20-21 22.11229427138518 27.518630359539447 22.369675778766688 27.999399590308684 22-23 22.57151686385308 30.976514215080346 22.474395196892107 23.977573724174466 24-25 25.713844252163163 29.521896521278475 23.168373653540524 21.595885573017835 26-27 24.973511804905613 30.97352946370234 23.926345158840874 20.126613572551165 28-29 21.84540811146085 28.23869079414972 26.871476564879764 23.044424529509666 30-31 21.84011054260349 26.26446346664077 29.015226095824193 22.88019989493155 32-33 24.682027080123436 26.836428814240243 24.343415168224343 24.13812893741198 34-35 27.982076637824477 26.61266113367473 24.559862263817763 20.845399964683033 36-37 23.4645947377715 28.88839837541939 23.92636411795868 23.720642768850432 38-39 21.247759559946672 28.77431374107135 27.19254099011999 22.785385708861988 40-41 21.389123112172594 28.08177752663156 27.374987307795884 23.154112053399967 42-43 24.10667773294366 26.583345841758128 27.601825935703754 21.708150489594463 44-45 20.503900438385436 29.442901731909426 28.94447559301232 21.108722236692817 46-47 22.261758655094166 25.963958077646414 28.305976672523553 23.468306594735864 48-49 20.755783153805403 25.49355465301077 27.34725410559774 26.403408087586083 50-51 19.433150122508444 28.112487031763898 29.146414144758626 23.307948700969032 52-53 17.87893235857636 29.22240175173717 30.554304735164532 22.344361154521934 54-55 16.771664319487183 30.411499560731613 29.11532671414003 23.70150940564118 56-57 19.29872583906544 28.304385833237678 27.431104361109394 24.96578396658749 58-59 16.914845243403363 28.874212531509773 29.54833412061788 24.66260810446898 60-61 19.287565281499568 29.337053403908726 27.55486294747902 23.82051836711269 62-63 16.72310049046658 34.07043117795858 24.540987731713454 24.665480599861382 64-65 17.01346119035951 34.76466095371805 23.139207869211447 25.082669986710993 66-67 17.124618128520723 34.44878066008582 22.612530682159317 25.81407052923414 68-69 17.20880129971039 34.73237620964893 22.128628946810768 25.930193543829905 70-71 18.871989580803955 33.41780534646034 21.93638109534006 25.773823977395644 72-73 25.102088566522013 26.513007738865173 22.039652302897327 26.345251391715486 74-75 26.989859212135393 24.840294731823178 21.77333551129967 26.39651054474176 76 27.746971355914248 23.817680923957194 21.82654610956098 26.608801610567586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.0 14 4.5 15 9.0 16 14.5 17 18.0 18 27.0 19 39.0 20 70.0 21 98.0 22 98.0 23 125.0 24 190.5 25 229.0 26 323.5 27 502.5 28 675.0 29 763.0 30 939.0 31 1268.5 32 1640.5 33 1859.0 34 2120.5 35 2765.0 36 3520.0 37 3892.0 38 4251.5 39 5018.0 40 5799.0 41 6551.0 42 6929.0 43 7202.0 44 7699.0 45 7978.0 46 8033.0 47 8030.5 48 7712.5 49 6989.0 50 6581.0 51 6010.0 52 4878.5 53 3929.0 54 3540.0 55 3238.0 56 2644.5 57 2065.0 58 1777.0 59 1552.5 60 1197.0 61 926.0 62 786.0 63 697.5 64 563.0 65 467.0 66 414.0 67 411.0 68 385.0 69 335.5 70 282.5 71 253.0 72 236.5 73 195.0 74 173.5 75 177.0 76 161.5 77 147.0 78 136.0 79 124.0 80 106.5 81 86.0 82 75.5 83 68.0 84 57.5 85 35.5 86 27.0 87 30.0 88 26.0 89 19.0 90 12.5 91 10.0 92 11.0 93 10.0 94 7.5 95 11.5 96 17.0 97 18.5 98 35.0 99 273.0 100 496.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007063393960798163 2 8.829242450997703E-4 3 0.0 4 0.0 5 0.0 6 0.0035316969803990813 7 8.829242450997703E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0026487727352993116 14-15 0.0 16-17 0.0013243863676496558 18-19 0.0 20-21 0.0035316969803990813 22-23 0.0 24-25 0.0 26-27 0.0017658484901995407 28-29 4.4146212254988517E-4 30-31 4.4146212254988517E-4 32-33 0.003090234857849197 34-35 0.0 36-37 0.0 38-39 8.829242450997703E-4 40-41 0.0013243863676496558 42-43 0.0026487727352993116 44-45 0.003090234857849197 46-47 0.0026487727352993116 48-49 0.0 50-51 0.002207310612749426 52-53 8.829242450997703E-4 54-55 0.003090234857849197 56-57 0.007946318205897933 58-59 0.0013243863676496558 60-61 4.4146212254988517E-4 62-63 4.4146212254988517E-4 64-65 0.007504856083348049 66-67 0.0017658484901995407 68-69 0.0035316969803990813 70-71 0.006621931838248278 72-73 4.4146212254988517E-4 74-75 0.003973159102948967 76 0.007063393960798163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 113260.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.77291188416034 #Duplication Level Percentage of deduplicated Percentage of total 1 89.80413751403646 73.4354582376832 2 6.047551179061933 9.89051739360763 3 1.9910166709855748 4.884336923891929 4 0.8659410900924246 2.8324209782800636 5 0.43081109095620623 1.761433868974042 6 0.27748985056577696 1.361469185943846 7 0.1619590567504535 0.9270704573547589 8 0.09933488814027813 0.649832244393431 9 0.08853761769024791 0.6515980928836306 >10 0.2278224064956379 2.619636235211019 >50 0.0021594540900060463 0.10506798516687267 >100 0.0021594540900060463 0.4185060921772911 >500 0.0010797270450030232 0.46265230443227967 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 524 0.46265230443227967 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT 351 0.30990641003001945 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG 123 0.10859968214727177 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.829242450997704E-4 0.0 5 0.0 0.0 0.0 0.002648772735299311 0.0 6 0.0 0.0 0.0 0.002648772735299311 0.0 7 0.0 0.0 0.0 0.002648772735299311 0.0 8 0.0 0.0 0.0 0.002648772735299311 0.0 9 0.0 0.0 0.0 0.005297545470598622 0.0 10 0.0 0.0 0.0 0.006180469715698393 0.0 11 0.0 0.0 0.0 0.006180469715698393 0.0 12 0.0 0.0 0.0 0.006180469715698393 0.0 13 0.0 0.0 0.0 0.006180469715698393 0.0 14 0.0 0.0 0.0 0.0070633939607981635 0.0 15 0.0 0.0 0.0 0.0070633939607981635 0.0 16 0.0 0.0 0.0 0.0070633939607981635 0.0 17 0.0 0.0 0.0 0.007946318205897933 0.0 18 0.0 0.0 0.0 0.013243863676496557 0.0 19 0.0 0.0 0.0 0.02030725763729472 0.0 20 0.0 0.0 0.0 0.025604803107893342 0.0 21 0.0 0.0 0.0 0.027370651598092884 0.0 22 0.0 0.0 0.0 0.029136500088292425 0.0 23 0.0 0.0 0.0 0.029136500088292425 0.0 24 0.0 0.0 0.0 0.035316969803990815 0.0 25 0.0 0.0 0.0 0.04061451527458944 0.0 26 0.0 0.0 0.0 0.0476779092353876 0.0 27 0.0 0.0 0.0 0.07063393960798163 0.0 28 0.0 0.0 0.0 0.09712166696097475 0.0 29 0.0 0.0 0.0 0.14391665195126258 0.0 30 0.0 0.0 0.0 0.18453116722585203 0.0 31 0.0 0.0 0.0 0.22337983401024192 0.0 32 0.0 0.0 0.0 0.29401377361822356 0.0 33 0.0 0.0 0.0 0.36199894049090586 0.0 34 0.0 0.0 0.0 0.43793042556948614 0.0 35 0.0 0.0 0.0 0.5791983047854494 0.0 36 0.0 0.0 0.0 0.7911001236093943 0.0 37 0.0 0.0 0.0 1.1751721702277944 0.0 38 0.0 0.0 0.0 1.707575490022956 0.0 39 0.0 0.0 0.0 2.3300370828182944 0.0 40 0.0 0.0 0.0 3.1811760550944728 0.0 41 0.0 0.0 0.0 4.140031785272823 0.0 42 0.0 0.0 0.0 5.242804167402437 0.0 43 0.0 0.0 0.0 6.339396079816352 0.0 44 0.0 0.0 0.0 7.356524810171288 0.0 45 0.0 0.0 0.0 8.370121843545824 0.0 46 0.0 0.0 0.0 9.389899346636058 0.0 47 0.0 0.0 0.0 10.444110895285185 0.0 48 0.0 0.0 0.0 11.5380540349638 0.0 49 0.0 0.0 0.0 12.605509447289423 0.0 50 0.0 0.0 0.0 13.568779798693273 0.0 51 0.0 0.0 0.0 14.584142680558008 0.0 52 0.0 0.0 0.0 15.620695744305138 0.0 53 0.0 0.0 0.0 16.602507504856085 0.0 54 0.0 0.0 0.0 17.53664135617164 0.0 55 0.0 0.0 0.0 18.469009358997 0.0 56 0.0 0.0 0.0 19.291011831184885 0.0 57 0.0 0.0 0.0 20.172170227794457 0.0 58 0.0 0.0 0.0 20.96415327564895 0.0 59 0.0 0.0 0.0 21.716404732473954 0.0 60 0.0 0.0 0.0 22.453646477132263 0.0 61 0.0 0.0 0.0 23.240331979516156 0.0 62 0.0 0.0 0.0 23.99964683030196 0.0 63 0.0 0.0 0.0 24.71923009005827 0.0 64 0.0 0.0 0.0 25.426452410383188 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 30 1.01596015E-7 70.00001 31 CGTAAGA 15 0.0022197147 70.00001 41 TCGAGGC 15 0.0022197147 70.00001 31 TCGACTA 15 0.0022197147 70.00001 39 ATCGACT 15 0.0022197147 70.00001 38 TGCCGAA 15 0.0022197147 70.00001 45 CGACACT 30 8.331615E-6 58.333336 23 TACGCGG 30 8.331615E-6 58.333336 29 GCCGAAA 25 2.3818132E-4 55.999996 46 ATGACGA 25 2.3818132E-4 55.999996 44 CTATGCA 20 0.0069359457 52.500004 34 GCGCCTT 20 0.0069359457 52.500004 30 TTCGTTC 20 0.0069359457 52.500004 40 CGTCTTA 40 7.4240415E-7 52.500004 48 AGAACGG 20 0.0069359457 52.500004 27 GTGTGTC 20 0.0069359457 52.500004 36 AATCTCG 20 0.0069359457 52.500004 36 AACTCGT 20 0.0069359457 52.500004 37 GAATTAC 20 0.0069359457 52.500004 42 CCGTCTT 75 0.0 51.333332 47 >>END_MODULE