FastQCFastQC Report
Wed 25 May 2016
SRR1780817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7303
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394253.97781733534164No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG2012.7522935779816518No Hit
TCTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGT290.3970970833903876No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.17800903738189786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG120.16431603450636723No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.13693002875530602No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.12323702587977543No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGG90.12323702587977543No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.10954402300424482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG80.10954402300424482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGT252.1703313E-456.33655533
GGCTGCG252.1703313E-456.33655530
CTGCGTG252.1703313E-456.33655532
GCTGCGT252.1703313E-456.33655531
AGGCTGC252.1703313E-456.33655529
GTAGATC252.246378E-455.95068738
ATCTCGG252.246378E-455.95068742
GTGTAGA252.246378E-455.95068736
ACGAGAG252.246378E-455.95068724
GATCTCG252.246378E-455.95068741
TGTAGAT252.246378E-455.95068737
GACGAGA252.246378E-455.95068723
CGACGAG252.246378E-455.95068722
CTGCCGA252.246378E-455.95068718
AAAAAGG252.246378E-455.95068770
AGATCTC252.246378E-455.95068740
CCGACGA252.246378E-455.95068721
TAGATCT252.246378E-455.95068739
TCGGTGG351.8988298E-549.95596745
CGGTGGT351.8988298E-549.95596746
Produced by FastQC (version 0.11.5)