FastQCFastQC Report
Wed 25 May 2016
SRR1780817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7303
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4055.545666164589894No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG2773.7929617965219773No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2383.2589346843762836No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT1942.6564425578529374Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC1522.081336437080652No Hit
ATACCCATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT1071.4651513076817746Illumina PCR Primer Index 11 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT921.2597562645488156No Hit
TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG871.1912912501711626No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC660.90373818978502No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC600.8215801725318362No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC510.6983431466520608No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT440.6024921265233465Illumina PCR Primer Index 11 (95% over 21bp)
CATAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT400.5477201150212241No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT340.46556209776804053No Hit
CAAAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT330.45186909489251No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG310.4244830891414487No Hit
TCATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC300.4107900862659181No Hit
TCTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG290.3970970833903876No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT250.3423250718882651No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG240.32863206901273445No Hit
AAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT220.30124606326167325Illumina PCR Primer Index 11 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC210.2875530603861427No Hit
TCTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCG210.2875530603861427No Hit
CAAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT210.2875530603861427No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAGCTCCATCTCGTATGCCGT190.2601670546350815No Hit
TCTTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCC160.21908804600848963No Hit
TCTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT160.21908804600848963No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT150.20539504313295906No Hit
TCATAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG150.20539504313295906No Hit
CTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCGT130.17800903738189786No Hit
TCATAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC130.17800903738189786No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGTCTT110.15062303163083662No Hit
AAACCCATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT100.13693002875530602Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCC100.13693002875530602No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCAACTCGTATGCCG100.13693002875530602No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAG100.13693002875530602No Hit
CTAAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT100.13693002875530602No Hit
TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAG90.12323702587977543No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCC90.12323702587977543No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG90.12323702587977543No Hit
CTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGTC90.12323702587977543No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGAC90.12323702587977543No Hit
CATATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT90.12323702587977543No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA80.10954402300424482No Hit
TCATATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCG80.10954402300424482No Hit
TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACG80.10954402300424482No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACG80.10954402300424482No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCACCATCTCGTATGCCGT80.10954402300424482No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCAT80.10954402300424482No Hit
CAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC80.10954402300424482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAC252.5682803E-670.0000145
ATGCCGA252.5682803E-670.0000144
TCTTTAT500.070.000011
CCAACAC150.002137153470.035
ACCATCT150.002137153470.034
TAAAAAC207.4136035E-570.03
GAGCCGA207.4136035E-570.026
CACGAGC150.002137153470.023
AAGGGGC150.002137153470.070
GACGCAC150.002137153470.029
CACCATC150.002137153470.033
CGAGCCG207.4136035E-570.025
TACCCAT150.002137153470.02
ATACCCA150.002137153470.01
AGCCGAC207.4136035E-570.027
GCCGACT150.002137153470.046
GCCGACG207.4136035E-570.028
CCGACGC207.4136035E-570.029
TCATATA353.074092E-970.01
CTTTATA550.063.6363682
Produced by FastQC (version 0.11.5)