##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7303 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.619882240175272 32.0 27.0 32.0 13.0 32.0 2 27.133232917978912 32.0 27.0 32.0 13.0 32.0 3 26.24127071066685 32.0 22.0 32.0 13.0 32.0 4 29.02683828563604 32.0 27.0 32.0 22.0 32.0 5 25.04546076954676 27.0 22.0 32.0 8.0 32.0 6 27.228810078050117 32.0 13.0 37.0 8.0 37.0 7 31.46474051759551 37.0 27.0 37.0 13.0 37.0 8 30.598247295631932 37.0 27.0 37.0 13.0 37.0 9 32.519649459126384 37.0 32.0 37.0 22.0 37.0 10-11 31.002464740517595 37.0 27.0 37.0 13.0 37.0 12-13 31.841435026701355 37.0 29.5 37.0 17.5 37.0 14-15 32.096741065315626 37.0 29.5 37.0 13.0 37.0 16-17 29.451047514719978 37.0 24.5 37.0 10.5 37.0 18-19 29.254279063398602 37.0 20.0 37.0 10.5 37.0 20-21 32.27255922223743 37.0 32.0 37.0 13.0 37.0 22-23 30.885800356018073 37.0 29.5 37.0 13.0 37.0 24-25 29.926879364644666 37.0 24.5 37.0 10.5 37.0 26-27 29.830412159386555 37.0 24.5 37.0 13.0 37.0 28-29 30.653430097220323 37.0 29.5 37.0 13.0 37.0 30-31 30.04429686430234 37.0 27.0 37.0 13.0 37.0 32-33 29.839791866356293 37.0 24.5 37.0 10.5 37.0 34-35 29.816034506367245 37.0 24.5 37.0 8.0 37.0 36-37 29.081267972066275 37.0 22.0 37.0 10.5 37.0 38-39 26.842735861974532 32.0 13.0 37.0 8.0 37.0 40-41 28.412570176639736 34.5 17.5 37.0 8.0 37.0 42-43 28.95008900451869 37.0 22.0 37.0 8.0 37.0 44-45 30.18252772833082 37.0 27.0 37.0 13.0 37.0 46-47 28.303505408736136 37.0 17.5 37.0 8.0 37.0 48-49 27.327673558811448 34.5 17.5 37.0 10.5 37.0 50-51 28.033616322059427 34.5 17.5 37.0 10.5 37.0 52-53 28.645214295495002 34.5 22.0 37.0 13.0 37.0 54-55 28.734492674243462 34.5 22.0 37.0 13.0 37.0 56-57 26.45892099137341 32.0 13.0 37.0 8.0 37.0 58-59 28.095782555114337 32.0 17.5 37.0 13.0 37.0 60-61 27.439066137203888 34.5 17.5 37.0 8.0 37.0 62-63 30.19512529097631 37.0 27.0 37.0 13.0 37.0 64-65 30.668492400383403 37.0 27.0 37.0 13.0 37.0 66-67 30.166233054908943 37.0 27.0 37.0 13.0 37.0 68-69 29.110365603176774 37.0 22.0 37.0 13.0 37.0 70-71 26.68492400383404 29.5 13.0 37.0 10.5 37.0 72-73 24.694166780775024 29.5 13.0 37.0 8.0 37.0 74-75 27.073326030398466 34.5 13.0 37.0 8.0 37.0 76 28.429823360262905 37.0 13.0 37.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 11101 1 1.7522238756613824 11101 2 5.026143628747807 11101 3 -3.1213293650793688 11101 4 -8.746913580246908 11101 5 7.674809854497347 11101 6 -4.781362985008805 11101 7 0.43631227954145047 11101 8 3.5016038359788304 11101 9 -7.638188932980597 11101 10-11 -3.681823192239861 11101 12-13 -10.365543705908287 11101 14-15 5.052291391093483 11101 16-17 -4.787436618165785 11101 18-19 1.7713583002645485 11101 20-21 -6.648357583774242 11101 22-23 -5.600555279982352 11101 24-25 4.580324074074081 11101 26-27 -5.1864197530864224 11101 28-29 -10.8610298170194 11101 30-31 -4.344693838183407 11101 32-33 -6.336531360229269 11101 34-35 -3.1525958994708887 11101 36-37 0.8238425925926052 11101 38-39 2.0084270282186907 11101 40-41 2.4143546075837747 11101 42-43 5.206106701940037 11101 44-45 0.6628692680776176 11101 46-47 -1.3754092261904702 11101 48-49 -7.270425485008815 11101 50-51 -4.250192901234566 11101 52-53 2.762870645943572 11101 54-55 -11.93334297839506 11101 56-57 -15.245603229717808 11101 58-59 -5.6084793871252 11101 60-61 0.960850694444435 11101 62-63 -5.138454861111125 11101 64-65 -6.467252259700167 11101 66-67 3.4095610119047777 11101 68-69 -1.4503734016754741 11101 70-71 -0.4010802469135939 11101 72-73 -0.8080770502645649 11101 74-75 -3.7014936067019413 11101 76 8.260422178130494 11102 1 4.252223875661382 11102 2 5.026143628747807 11102 3 6.378670634920631 11102 4 3.2530864197530924 11102 5 -16.325190145502653 11102 6 9.718637014991195 11102 7 5.4363122795414505 11102 8 6.00160383597883 11102 9 4.361811067019403 11102 10-11 3.568176807760139 11102 12-13 5.384456294091713 11102 14-15 5.052291391093483 11102 16-17 7.462563381834215 11102 18-19 -6.7286416997354515 11102 20-21 4.351642416225758 11102 22-23 6.399444720017648 11102 24-25 7.080324074074081 11102 26-27 4.3135802469135776 11102 28-29 6.1389701829806 11102 30-31 4.155306161816593 11102 32-33 6.913468639770731 11102 34-35 7.847404100529111 11102 36-37 8.073842592592605 11102 38-39 4.508427028218691 11102 40-41 5.914354607583775 11102 42-43 5.206106701940037 11102 44-45 6.662869268077618 11102 46-47 3.3745907738095298 11102 48-49 9.729574514991185 11102 50-51 6.499807098765434 11102 52-53 6.262870645943572 11102 54-55 8.56665702160494 11102 56-57 -6.995603229717808 11102 58-59 -1.1084793871252003 11102 60-61 -5.039149305555565 11102 62-63 6.861545138888875 11102 64-65 6.782747740299833 11102 66-67 7.159561011904778 11102 68-69 8.299626598324526 11102 70-71 -1.401080246913594 11102 72-73 -7.058077050264565 11102 74-75 4.548506393298059 11102 76 8.260422178130494 11103 1 2.5855572089947145 11103 2 5.026143628747807 11103 3 6.378670634920631 11103 4 -9.413580246913575 11103 5 -8.325190145502653 11103 6 -7.948029651675473 11103 7 -5.897021053791882 11103 8 -1.9983961640211696 11103 9 -10.304855599647265 11103 10-11 3.568176807760139 11103 12-13 -6.782210372574955 11103 14-15 1.0522913910934832 11103 16-17 -1.7041032848324527 11103 18-19 6.104691633597877 11103 20-21 0.18497574955909357 11103 22-23 4.732778053350977 11103 24-25 5.41365740740741 11103 26-27 2.8135802469135776 11103 28-29 0.3056368496472679 11103 30-31 4.155306161816593 11103 32-33 -1.2531980268959337 11103 34-35 0.5140707671957792 11103 36-37 5.573842592592605 11103 38-39 3.0084270282186907 11103 40-41 3.581021274250439 11103 42-43 2.0394400352733726 11103 44-45 -2.170464065255718 11103 46-47 5.041257440476201 11103 48-49 -0.7704254850088148 11103 50-51 -6.333526234567902 11103 52-53 -5.737129354056428 11103 54-55 -4.266676311728396 11103 56-57 -0.49560322971780835 11103 58-59 0.058187279541463965 11103 60-61 -6.872482638888897 11103 62-63 1.0282118055555394 11103 64-65 -2.2172522597001674 11103 66-67 3.826227678571449 11103 68-69 -6.200373401675474 11103 70-71 4.098919753086406 11103 72-73 0.2752562830687708 11103 74-75 -5.784826940035277 11103 76 6.593755511463829 11104 1 4.252223875661382 11104 2 0.026143628747806957 11104 3 -15.121329365079369 11104 4 -6.246913580246908 11104 5 -4.325190145502653 11104 6 -2.781362985008805 11104 7 5.4363122795414505 11104 8 -1.4983961640211696 11104 9 -0.6381889329805972 11104 10-11 4.818176807760139 11104 12-13 4.134456294091713 11104 14-15 2.5522913910934832 11104 16-17 7.462563381834215 11104 18-19 1.7713583002645485 11104 20-21 4.351642416225758 11104 22-23 -2.100555279982352 11104 24-25 4.580324074074081 11104 26-27 -6.4364197530864224 11104 28-29 2.3889701829806 11104 30-31 4.155306161816593 11104 32-33 -0.5865313602292694 11104 34-35 1.8474041005291113 11104 36-37 3.073842592592605 11104 38-39 -6.241572971781309 11104 40-41 2.1643546075837747 11104 42-43 0.4561067019400369 11104 44-45 6.662869268077618 11104 46-47 1.1245907738095298 11104 48-49 4.729574514991185 11104 50-51 5.249807098765434 11104 52-53 0.2628706459435719 11104 54-55 -4.933342978395061 11104 56-57 2.7543967702821917 11104 58-59 7.6415206128748 11104 60-61 3.210850694444435 11104 62-63 5.611545138888875 11104 64-65 -5.217252259700167 11104 66-67 7.159561011904778 11104 68-69 5.799626598324526 11104 70-71 3.348919753086406 11104 72-73 -3.308077050264565 11104 74-75 2.2985063932980587 11104 76 5.760422178130494 11105 1 -7.08110945767195 11105 2 -4.640523037918861 11105 3 -6.287996031746037 11105 4 -3.0802469135802397 11105 5 -6.658523478835985 11105 6 -9.614696318342137 11105 7 -4.230354387125217 11105 8 1.0016038359788304 11105 9 -0.6381889329805972 11105 10-11 -2.7651565255731967 11105 12-13 -0.28221037257495496 11105 14-15 -1.4477086089065168 11105 16-17 0.1292300485008795 11105 18-19 6.93802496693122 11105 20-21 1.0183090828924222 11105 22-23 3.0661113866843195 11105 24-25 -9.919675925925919 11105 26-27 -3.6864197530864224 11105 28-29 0.4723035163139322 11105 30-31 -6.178027171516742 11105 32-33 0.41346863977073056 11105 34-35 -0.9859292328042208 11105 36-37 0.7405092592592695 11105 38-39 5.341760361552026 11105 40-41 -2.0856453924162253 11105 42-43 -5.960559964726627 11105 44-45 -2.170464065255718 11105 46-47 5.041257440476201 11105 48-49 0.06290784832451735 11105 50-51 0.666473765432098 11105 52-53 -0.9037960207230924 11105 54-55 2.899990354938275 11105 56-57 -2.162269896384476 11105 58-59 -10.441812720458536 11105 60-61 -5.039149305555565 11105 62-63 -9.30512152777779 11105 64-65 -6.883918926366832 11105 66-67 -7.340438988095222 11105 68-69 -10.367040068342142 11105 70-71 -3.901080246913594 11105 72-73 1.1085896164020994 11105 74-75 2.215173059964723 11105 76 -1.4062444885361742 11106 1 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64-65 2.639890597442694 21612 66-67 0.23098958333334707 21612 68-69 -1.34323054453262 21612 70-71 2.9560626102292638 21612 72-73 -1.4866484788359955 21612 74-75 0.8342206790123434 21612 76 -2.1681492504409334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 3.0 18 7.0 19 33.0 20 82.0 21 172.0 22 271.0 23 379.0 24 425.0 25 471.0 26 472.0 27 532.0 28 532.0 29 563.0 30 598.0 31 690.0 32 678.0 33 644.0 34 484.0 35 218.0 36 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.006709571409008 21.100917431192663 26.55073257565384 35.34164042174449 2 11.981377516089278 30.247843352047106 28.714227029987676 29.056552101875944 3 11.419964398192525 42.70847596877995 39.545392304532385 6.3261673284951385 4 10.844858277420238 41.73627276461728 32.986443927153225 14.432425030809256 5 10.105436122141585 52.54005203341092 26.851978638915515 10.502533205531973 6 12.00876352184034 52.54005203341092 17.2257976174175 18.22538682733123 7 12.666027659865808 40.188963439682325 11.529508421196768 35.615500479255104 8 10.954402300424483 49.58236341229632 11.967684513213747 27.495549774065452 9 11.159797343557443 28.125427906339862 5.65521018759414 55.059564562508555 10-11 11.447350403943584 37.9638504724086 25.19512529097631 25.393673832671503 12-13 15.863343831302204 15.212926194714502 32.45241681500753 36.47131315897577 14-15 18.06791729426263 13.617691359715186 16.486375462138845 51.82801588388334 16-17 37.58729289333151 23.640969464603586 5.059564562508558 33.712173079556344 18-19 39.10036971107764 24.072299055182803 5.107490072572915 31.719841161166645 20-21 19.690538135013007 12.001917020402574 4.525537450362864 63.78200739422155 22-23 18.198000821580173 36.57401068054224 4.546076954676161 40.68191154320143 24-25 37.51198137751609 34.239353690264274 5.0527180610707925 23.19594687114884 26-27 44.1599342735862 41.48979871285773 5.401889634396824 8.94837737915925 28-29 36.88210324524168 22.949472819389293 5.579898671778722 34.588525263590306 30-31 26.420649048336298 30.535396412433247 9.475557989867179 33.56839654936328 32-33 33.27399698753937 13.213747774887032 8.763521840339585 44.74873339723401 34-35 15.130768177461318 15.630562782418183 31.35013008352732 37.88853895659318 36-37 11.125564836368616 26.96152266191976 16.19197590031494 45.72093660139669 38-39 15.486786252225112 25.448445844173627 28.08434889771327 30.980419005887995 40-41 13.508147336710941 16.561686977954267 35.26632890592907 34.66383677940572 42-43 37.95700397097083 12.91250171162536 30.63124743256196 18.499246884841845 44-45 17.841982746816377 34.472134739148295 33.89018211693824 13.795700397097082 46-47 38.039161988224016 14.829522114199644 22.51129672737231 24.620019170204024 48-49 31.13788853895659 11.406271395316994 12.618102149801452 44.83773791592496 50-51 22.579761741749966 13.14528276050938 34.00657264138026 30.268382856360397 52-53 14.576201561002328 16.19197590031494 49.212652334656994 20.019170204025745 54-55 12.947620677850052 15.261896610749742 39.46593632317699 32.32454638822321 56-57 35.78666301519923 12.337395590853074 24.52416815007531 27.35177324387238 58-59 17.040942078597837 25.27043680679173 40.209502943995616 17.479118170614814 60-61 32.41133780638094 40.09995892099138 21.121456935505954 6.36724633712173 62-63 14.001095440230044 74.31192660550458 5.792140216349445 5.894837737915925 64-65 10.043817609201698 79.22771463782009 4.689853484869231 6.038614268108996 66-67 10.358756675338903 79.07024510475146 4.607695467616049 5.963302752293578 68-69 17.97206627413392 71.2104614541969 4.710392989182528 6.10707928248665 70-71 30.564875042793567 57.02156795617939 5.114686751112632 7.298870249914414 72-73 68.64986991647268 17.95152676982062 4.826783513624537 8.571819800082157 74-75 78.14596741065316 8.270573736820484 5.032178556757497 8.551280295768862 76 80.66547993975078 7.366835547035465 4.641927974804875 7.325756538408873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 2.0 14 1.0 15 0.5 16 1.0 17 1.0 18 0.5 19 4.0 20 6.5 21 5.0 22 5.0 23 6.0 24 9.0 25 11.0 26 15.5 27 17.5 28 19.0 29 23.0 30 36.0 31 49.5 32 46.0 33 42.0 34 54.0 35 73.0 36 83.5 37 87.0 38 93.0 39 111.0 40 136.5 41 153.0 42 156.0 43 182.5 44 230.0 45 305.5 46 360.0 47 445.5 48 736.5 49 1066.0 50 1190.0 51 1033.0 52 703.0 53 454.0 54 378.0 55 311.0 56 160.0 57 66.0 58 56.0 59 52.5 60 40.0 61 27.5 62 24.0 63 24.0 64 16.0 65 9.0 66 10.0 67 10.0 68 8.5 69 7.5 70 8.0 71 8.0 72 7.0 73 4.0 74 2.0 75 2.0 76 1.0 77 1.0 78 1.0 79 0.0 80 3.5 81 3.5 82 1.5 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.5 89 1.5 90 1.0 91 1.0 92 2.0 93 1.0 94 1.0 95 6.0 96 10.0 97 12.0 98 25.5 99 212.5 100 388.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.006846501437765301 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.006846501437765301 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.892783787484596 #Duplication Level Percentage of deduplicated Percentage of total 1 92.53429278165055 56.34670683280844 2 3.5979311895659998 4.381760920169793 3 1.2367888464133123 2.2593454744625494 4 0.7420733078479874 1.80747637957004 5 0.44974139869574997 1.3693002875530604 6 0.2023836294130875 0.7394221552786526 7 0.11243534967393749 0.47925510064357113 8 0.15740948954351247 0.7668081610297138 9 0.13492241960872497 0.7394221552786526 >10 0.5846638183044749 7.435300561413118 >50 0.11243534967393749 4.8747090236888955 >100 0.13492241960872497 18.80049294810352 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 405 5.545666164589894 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 277 3.7929617965219773 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 238 3.2589346843762836 No Hit ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT 194 2.6564425578529374 Illumina PCR Primer Index 11 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC 152 2.081336437080652 No Hit ATACCCATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT 107 1.4651513076817746 Illumina PCR Primer Index 11 (95% over 22bp) CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 92 1.2597562645488156 No Hit TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 87 1.1912912501711626 No Hit CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC 66 0.90373818978502 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC 60 0.8215801725318362 No Hit CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC 51 0.6983431466520608 No Hit TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT 44 0.6024921265233465 Illumina PCR Primer Index 11 (95% over 21bp) CATAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 40 0.5477201150212241 No Hit CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 34 0.46556209776804053 No Hit CAAAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 33 0.45186909489251 No Hit CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 31 0.4244830891414487 No Hit TCATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC 30 0.4107900862659181 No Hit TCTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 29 0.3970970833903876 No Hit CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 25 0.3423250718882651 No Hit CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 24 0.32863206901273445 No Hit AAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT 22 0.30124606326167325 Illumina PCR Primer Index 11 (95% over 22bp) TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC 21 0.2875530603861427 No Hit TCTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCG 21 0.2875530603861427 No Hit CAAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 21 0.2875530603861427 No Hit CTTATACACATCTCCGAGCCCACGAGACGAAGCTCCATCTCGTATGCCGT 19 0.2601670546350815 No Hit TCTTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCC 16 0.21908804600848963 No Hit TCTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 16 0.21908804600848963 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT 15 0.20539504313295906 No Hit TCATAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 15 0.20539504313295906 No Hit CTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCGT 13 0.17800903738189786 No Hit TCATAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC 13 0.17800903738189786 No Hit ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGTCTT 11 0.15062303163083662 No Hit AAACCCATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT 10 0.13693002875530602 Illumina PCR Primer Index 11 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCC 10 0.13693002875530602 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCAACTCGTATGCCG 10 0.13693002875530602 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAG 10 0.13693002875530602 No Hit CTAAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 10 0.13693002875530602 No Hit TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAG 9 0.12323702587977543 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCC 9 0.12323702587977543 No Hit TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG 9 0.12323702587977543 No Hit CTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGTC 9 0.12323702587977543 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGAC 9 0.12323702587977543 No Hit CATATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT 9 0.12323702587977543 No Hit GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA 8 0.10954402300424482 No Hit TCATATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCG 8 0.10954402300424482 No Hit TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACG 8 0.10954402300424482 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACG 8 0.10954402300424482 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCACCATCTCGTATGCCGT 8 0.10954402300424482 No Hit ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCAT 8 0.10954402300424482 No Hit CAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC 8 0.10954402300424482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.054772011502122415 0.0 20 0.0 0.0 0.0 0.06846501437765302 0.0 21 0.0 0.0 0.0 0.06846501437765302 0.0 22 0.0 0.0 0.0 0.08215801725318363 0.0 23 0.0 0.0 0.0 0.08215801725318363 0.0 24 0.0 0.0 0.0 0.09585102012871423 0.0 25 0.0 0.0 0.0 0.09585102012871423 0.0 26 0.0 0.0 0.0 0.09585102012871423 0.0 27 0.0 0.0 0.0 0.09585102012871423 0.0 28 0.0 0.0 0.0 0.10954402300424483 0.0 29 0.0 0.0 0.0 0.15062303163083665 0.0 30 0.0 0.0 0.0 0.17800903738189786 0.0 31 0.0 0.0 0.0 0.19170204025742846 0.0 32 0.0 0.0 0.0 0.21908804600848966 0.0 33 0.0 0.0 0.0 0.3012460632616733 0.0 34 0.0 0.0 0.0 0.3286320690127345 0.0 35 0.0 0.0 0.0 0.3423250718882651 0.0 36 0.0 0.0 0.0 0.3697110776393263 0.0 37 0.0 0.0 0.0 0.5614131178967547 0.0 38 0.0 0.0 0.0 0.5751061207722854 0.0 39 0.0 0.0 0.0 0.6983431466520608 0.0 40 0.0 0.0 0.0 0.8215801725318362 0.0 41 0.0 0.0 0.0 0.9585102012871423 0.0 42 0.0 0.0 0.0 1.1228262357935095 0.0 43 0.0 0.0 0.0 1.273449267424346 0.0 44 0.0 0.0 0.0 1.3556072846775298 0.0 45 0.0 0.0 0.0 1.58838833356155 0.0 46 0.0 0.0 0.0 1.725318362316856 0.0 47 0.0 0.0 0.0 1.7800903738189786 0.0 48 0.0 0.0 0.0 1.8759413939476928 0.0 49 0.0 0.0 0.0 2.0402574284540598 0.0 50 0.0 0.0 0.0 2.2730384773380803 0.0 51 0.0 0.0 0.0 2.3688894974667947 0.0 52 0.0 0.0 0.0 2.4784335204710395 0.0 53 0.0 0.0 0.0 2.560591537724223 0.0 54 0.0 0.0 0.0 2.7522935779816513 0.0 55 0.0 0.0 0.0 2.9576886211146105 0.0 56 0.0 0.0 0.0 3.0672326441188553 0.0 57 0.0 0.0 0.0 3.108311652745447 0.0 58 0.0 0.0 0.0 3.1904696699986306 0.0 59 0.0 0.0 0.0 3.286320690127345 0.0 60 0.0 0.0 0.0 3.40955771600712 0.0 61 0.0 0.0 0.0 3.4780227303847733 0.0 62 0.0 0.0 0.0 3.560180747637957 0.0 63 0.0 0.0 0.0 3.6149527591400794 0.0 64 0.0 0.0 0.0 3.697110776393263 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGAC 25 2.5682803E-6 70.00001 45 ATGCCGA 25 2.5682803E-6 70.00001 44 TCTTTAT 50 0.0 70.00001 1 CCAACAC 15 0.0021371534 70.0 35 ACCATCT 15 0.0021371534 70.0 34 TAAAAAC 20 7.4136035E-5 70.0 3 GAGCCGA 20 7.4136035E-5 70.0 26 CACGAGC 15 0.0021371534 70.0 23 AAGGGGC 15 0.0021371534 70.0 70 GACGCAC 15 0.0021371534 70.0 29 CACCATC 15 0.0021371534 70.0 33 CGAGCCG 20 7.4136035E-5 70.0 25 TACCCAT 15 0.0021371534 70.0 2 ATACCCA 15 0.0021371534 70.0 1 AGCCGAC 20 7.4136035E-5 70.0 27 GCCGACT 15 0.0021371534 70.0 46 GCCGACG 20 7.4136035E-5 70.0 28 CCGACGC 20 7.4136035E-5 70.0 29 TCATATA 35 3.074092E-9 70.0 1 CTTTATA 55 0.0 63.636368 2 >>END_MODULE