FastQCFastQC Report
Wed 25 May 2016
SRR1780816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31089
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1119035.99343819357329No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG2560.8234423751166007No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340.10936344044517353No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTT150.002204517170.044
TTCCTTT150.002204517170.045
GATCTCG452.5907866E-854.44444341
TGTGTTA200.006888821752.518
GGTGGTC505.9437298E-849.00000447
ATTAAAA751.2732926E-1146.66666862
ATCTCGG451.6330287E-646.66666442
TCGGTGG451.6330287E-646.66666445
CGTATCA604.6784407E-946.66666456
GTGGTCG451.6330287E-646.66666448
CATTAAA703.601599E-1045.00000461
TCATTAA703.601599E-1045.00000460
CCGTATC551.2582314E-744.54545255
CGGTGGT551.2582314E-744.54545246
GCCGTAT551.2582314E-744.54545254
AGATCTC551.2582314E-744.54545240
TAGATCT551.2582314E-744.54545239
TGGTCGC404.4918503E-543.7549
CTCGGTG404.4918503E-543.7544
GTGTAGA659.495125E-943.07692336