FastQCFastQC Report
Wed 25 May 2016
SRR1780816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31089
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT27278.771591238058479No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG11183.5961272475795294No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT6762.174402521792274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5291.701566470455788No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3080.9907041075621603No Hit
TCATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG2630.8459583775611953No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2570.8266589468943999No Hit
CATAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2330.7494612242272186No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT2160.6947795040046318TruSeq Adapter, Index 9 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2130.6851297886712342No Hit
CAAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1650.5307343433368716No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT1080.3473897520023159No Hit
CAAAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1070.3441731802245167No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT860.2766251728907331No Hit
TCTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG810.26054231400173694No Hit
ATACCCATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT720.23159316800154395TruSeq Adapter, Index 9 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC710.22837659622374473No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC670.21551030911254784No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC640.20586059377915017No Hit
CTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCGT550.17691144777895718No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC540.17369487600115796No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG470.15117887355656343No Hit
TCTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCG430.13831258644536654No Hit
TCATAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG410.13187944288976808No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG350.11258001222297276No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC350.11258001222297276No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT320.10293029688957508No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT320.10293029688957508No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT320.10293029688957508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG252.7763908E-670.0000143
ACTCGAA207.8079494E-570.038
CCATCAT150.002204517170.048
CTTATGC150.002204517170.051
GTCTTAT150.002204517170.049
TTGATGA150.002204517170.06
ACGACAT150.002204517170.047
TTCAGCA150.002204517170.052
CCGACTA207.8079494E-570.047
CGTCTTA150.002204517170.048
TCTTATG207.8079494E-570.050
AGGAGTA309.9182216E-870.028
ACGAGAA401.0224539E-861.2522
AAGCCGT401.0224539E-861.2544
CCCATCT352.8820614E-760.0000044
GAATGAA352.8820614E-760.00000456
TGCAGTC352.8820614E-760.00000445
TCTTTAT352.8820614E-760.0000041
ATCTGCA352.8820614E-760.00000452
CAGTCAT308.190464E-658.33333247
Produced by FastQC (version 0.11.5)