FastQCFastQC Report
Wed 25 May 2016
SRR1780815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415506
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264316.361159646310763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGC150.002224220769.9974756
AATGCGT200.006949920752.4981086
GCGCTAT602.5952068E-740.8318619
GACGCTC350.001255066739.99855463
GCCTTTA400.002416662435.002951
CGATATG400.002416662435.0029565
GCTTCTA708.676434E-735.0029451
TGATTCG400.002418089734.99873743
ATAACCG501.7089707E-434.9987345
GTTCGCA751.4878206E-632.6694235
GGCGCTA751.4892212E-632.6654918
TTGTACG450.004306087331.1099879
CGCGAGA450.004306087331.10998722
TCGCCGT703.486397E-529.99891552
GACGTCC703.486397E-529.9989156
GTCGCCG952.8181603E-729.47261851
TTCGGTC604.979935E-429.16561328
GGCGACG604.979935E-429.16561320
TCGGTCA604.979935E-429.16561329
TCGCAGC853.9484075E-628.82595837
Produced by FastQC (version 0.11.5)