FastQCFastQC Report
Wed 25 May 2016
SRR1780815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415506
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT89612.156647557435994No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG22820.5492098790390512No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT11000.2647374526480965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7110.17111666257526967No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5060.12177922821812442No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAT150.002223280370.0049410
CACGTAT401.3460522E-1069.9965139
GCCGTAA850.061.76162746
ACGTATG352.9723378E-759.9970140
TGCCGTA1950.053.8434745
AGGGGGG13150.051.6324170
GCCGTCA4400.050.9065546
AAGCCGT1250.050.39748444
TATGCCG21550.050.18311343
TGACGTA352.0857695E-549.9975145
ATCGTAT352.0857695E-549.9975139
ATGCCGT21000.049.8308544
GCCGTAT1200.049.58085646
CGTATGC21150.049.47743641
GCCGTCT15650.049.19882646
GTATGCC22350.049.16980742
TCGTATG21800.049.1259940
CCGTCTT15750.049.10866547
TGCCGTC19200.049.0340145
ATCTCGT23550.048.59630637
Produced by FastQC (version 0.11.5)