FastQCFastQC Report
Wed 25 May 2016
SRR1780814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8239
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123715.01395800461221No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG1842.233280737953635No Hit
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG260.3155722781891006No Hit
TCTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGT210.2548853016142736No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGG140.16992353440951571No Hit
CTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACAT130.1577861390945503No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC120.1456487437795849No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2304.7214903E-412.17391367
Produced by FastQC (version 0.11.5)