FastQCFastQC Report
Wed 25 May 2016
SRR1780814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8239
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5486.651292632601044No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT1371.662823158150261No Hit
ATACCCATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT770.9345794392523363No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC540.6554193470081321No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC330.4005340453938585No Hit
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT290.35198446413399687No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT270.32770967350406605No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC240.2912974875591698RNA PCR Primer, Index 19 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG190.23061051098434274No Hit
AAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT130.1577861390945503No Hit
TCTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT120.1456487437795849No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC110.13351134846461948No Hit
TCATAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC110.13351134846461948No Hit
AAACCCATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT100.12137395314965409No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG100.12137395314965409No Hit
TCATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC90.10923655783468868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC150.002146743770.08
CGCGATA150.002146743770.028
GCGATAA200.00670964352.529
CCCATCT200.00670964352.54
CCATCTC200.00670964352.55
ACCCATC200.00670964352.53
ACGCGAT200.00670964352.527
CCGTCTT754.9510134E-528.047
GCCGTCT754.9510134E-528.046
CGTCTTC754.9510134E-528.048
TGCCGTC905.2556825E-627.22222145
ATGCCGT905.2556825E-627.22222144
TATGCCG958.002751E-625.78947343
CTTCTGC958.002751E-625.78947348
AATCTCG1108.270108E-725.45454436
TATACAC700.001125780425.03
TTCTGCT1001.1918248E-524.49999849
GCTTGAA1001.1918248E-524.49999856
TTGAAAA1001.1918248E-524.49999858
CTTGAAA1001.1918248E-524.49999857
Produced by FastQC (version 0.11.5)