FastQCFastQC Report
Wed 25 May 2016
SRR1780813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220053
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171207.779943922600465No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG5100.23176234816157923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGC150.0022222570.0020454
GATCTCG1050.056.6683241
TCGCCGT1100.054.10478652
GCGGTTA200.00693756352.5134661
GGTCTAA200.00693756352.5134661
CGTATCG200.006943803352.50153450
GTTCCCG200.006943803352.50153454
GCCGTAT1200.052.50153454
TGGTGCC200.006950048352.48960520
ATCTCGG1200.049.5847842
TCTCGGT1300.048.4739743
TGGTCGC1400.047.51218849
GCCCAGT305.8615673E-446.678641
CTCGGTG1350.046.6786444
GTCGCCG1350.046.6680351
GTAGATC1350.046.6680338
CGCCGTA1350.044.0853853
TCGGTGG1600.043.76122345
AGATCTC1300.043.07818240
CGGTGGT1750.042.01077746
Produced by FastQC (version 0.11.5)