FastQCFastQC Report
Wed 25 May 2016
SRR1780813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220053
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT35541.6150654615024562No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG14890.676655169436454No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT9910.45034605299632363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9710.4412573334605754No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3770.1713223632488537No Hit
TCATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG3670.1667780034809796No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC3570.16223364371310547No Hit
CATAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3210.1458739485487587No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3010.1367852290130105No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT2720.1236065856861756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC150.002223423769.9927345
CGTAATC207.927393E-569.9768248
TTCGTAT200.00694121652.50647728
ATACCGT200.006947459752.49454544
GCCGTAA200.006947459752.49454546
CCATTCG200.006947459752.49454525
TACGACG200.00695370852.4826164
TCGAATG950.051.5853140
CCGACTT950.051.5735947
AACTCGT1050.050.00617237
TGCCGAC1400.049.99480445
AGGGGGG8750.048.7949370
CAGCTTG1300.048.48956754
AAACTCG1100.047.73316636
GACGACT305.8719935E-446.6618246
GCCGTCA1150.045.6474346
TATGACG851.8189894E-1245.28941343
AATCACG703.8198777E-1045.0055536
GCCGACT1250.044.79534546
TGACGTC551.3072167E-744.5408345