FastQCFastQC Report
Wed 25 May 2016
SRR1780812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62078
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3027348.766068494474695No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG3870.6234092593189214No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720.11598311801282257No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.10148522826121976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCT150.002214971570.07
CGCTGGG150.002214971570.016
GCACCGA150.002214971570.031
TGCAGCT150.002214971570.011
GCGCTGG252.8094255E-670.015
GCTCCTG150.002214971570.06
CGGTGGT900.054.44444346
GTAGATC850.053.52940838
GCGTGTA850.053.52940834
GGGTAGG200.006921237352.50000436
AAAAGGG200.006921237352.50000470
ATAGAAT200.006921237352.5000043
ACTAGTC200.006921237352.50000449
GGGAAAC200.006921237352.50000412
TGCTCCT200.006921237352.5000045
CGGCTGG200.006921237352.5000049
GGCGCTG407.369181E-752.50000414
AACTCCG200.006921237352.50000427
TCGGTGG800.052.50000445
GCCGTAT800.052.50000454
Produced by FastQC (version 0.11.5)