FastQCFastQC Report
Wed 25 May 2016
SRR1780812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62078
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1227119.767067237990915No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG28424.578111408228358No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT14682.364766906150327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7591.222655369051838No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT7051.135668030542221No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC4990.8038274428944231No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4520.7281162408582751No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC4370.7039530912722703No Hit
CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3760.6056896162891846No Hit
TCATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3220.5187022777795677No Hit
CATAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2570.4139952962402139No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT2240.3608363671510036TruSeq Adapter, Index 7 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1980.31895357453526213No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT1830.2947904249492574No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT1550.24968587905538192No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCG1450.23357711266471215No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT1330.21424659299590837No Hit
TCTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1080.17397467701923386No Hit
CTAAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1040.16753117046296595No Hit
ATACCCATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT1040.16753117046296595TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1020.16430941718483197No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC970.15625503398949708No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT960.1546441573504301No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT900.14497889751602822No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT890.14336802087696124No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC870.14014626759882728No Hit
CATATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT850.1369245143206933No Hit
CACTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC840.13531363768162633No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA830.13370276104255935No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT830.13370276104255935No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG760.12242662456909051No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCG730.11759399465188956No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC710.1143722413737556No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT710.1143722413737556No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG710.1143722413737556No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT660.10631785817842068No Hit
CAAAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT630.10148522826121976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA207.869748E-570.0000148
AAGGGTG207.869748E-570.0000170
CTCGAAA207.869748E-570.0000139
ACAACAG150.002214971570.050
GCCCAAG150.002214971570.018
TAACGTA150.002214971570.038
CAACAGC150.002214971570.051
CACGAAT150.002214971570.039
CCCAAGA150.002214971570.019
GTGTCAT150.002214971570.032
CAAATCG252.8094255E-670.036
ACTCCAG150.002214971570.012
GAACACG150.002214971570.018
TACAGCT150.002214971570.052
ACCTTGT150.002214971570.027
AAAGCCG150.002214971570.043
TCTTTAT900.069.999991
TCCGAGA700.065.013
AGGGGGG16950.064.8377570
GACCACG650.064.6153918
Produced by FastQC (version 0.11.5)