FastQCFastQC Report
Wed 25 May 2016
SRR1780811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59008
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4035568.38903199566161No Hit
CTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGTG6171.0456209327548807No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.21522505422993493No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG810.13726952277657267No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG770.13049078091106292No Hit
TCTTATACACATCTGACGCTGCCGACGAGAGGCTGCGTGTAGATCTCGGT760.12879609544468548No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750.12710140997830802No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.12032266811279828No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.11523861171366594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG680.11523861171366594No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG600.10168112798264642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1550.056.41060654
CCGTATC1500.055.95932455
TGGTCGC1600.054.6941349
GTCGCCG1600.054.6941351
CGCCGTA1600.054.6941353
GGTCGCC1600.054.6941350
AAAAAGG900.054.404970
CGTATCA1550.054.15418256
GTATCAT1500.053.67317257
ATCATTA1500.053.67317259
TCATTAA1500.053.67317260
GTGGTCG1700.053.53589648
TCGCCGT1650.053.03672852
TATCATT1600.052.50636358
AAAGGGG200.0069394752.46186469
GGTGGTC1750.052.00629847
ATCTCGG1700.051.4768242
TCTCGGT1700.051.4768243
GATCTCG1700.051.4768241
CTCGGTG1700.051.4768244