FastQCFastQC Report
Wed 25 May 2016
SRR1780811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59008
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1625227.54202819956616No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG39356.668587310195227No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT25554.32992136659436No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT12012.035317245119306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10911.8489018438177875No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT8991.5235222342733188No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC6221.0540943600867678No Hit
CATAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5800.9829175704989155No Hit
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT5610.950718546637744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC5590.9473291757049892No Hit
TCATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG5570.9439398047722344No Hit
CAAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4350.7371881778741866No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT3900.6609273318872017RNA PCR Primer, Index 19 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT2780.4711225596529284No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2360.3999457700650759No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT2330.39486171366594364No Hit
TCTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCG2250.3813042299349241No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1950.3304636659436008No Hit
TCTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1880.3186008676789588No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC1870.3169061822125814No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT1690.2864018438177874No Hit
ATACCCATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT1620.27453904555314534RNA PCR Primer, Index 19 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC1570.2660656182212581No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1350.22878253796095446No Hit
CATATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT1330.22539316702819956No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT1260.21353036876355747No Hit
CTTATACACATCACCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1040.17624728850325377No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC1030.17455260303687636RNA PCR Primer, Index 19 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC980.16607917570498915No Hit
CTTATCCACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT940.1593004338394794No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG930.15760574837310196No Hit
CTTAAACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT920.15591106290672452No Hit
TCATAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG880.14913232104121474No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG850.14404826464208242No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT840.142353579175705No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAG830.14065889370932755No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT820.1389642082429501RNA PCR Primer, Index 19 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC770.13049078091106292No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGA720.1220173535791757No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT670.1135439262472885No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT640.10845986984815618No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACG600.10168112798264642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC252.8077066E-670.0000151
ACAAATG252.8077066E-670.0000127
AACCGAG252.8077066E-670.0000112
ACTGATT252.8077066E-670.0000153
TACAGCT252.8077066E-670.0000152
TATGCCC252.8077066E-670.0000143
TTCTGAA252.8077066E-670.0000152
TAATGCT252.8077066E-670.0000152
TGCCCTC252.8077066E-670.0000145
TCAGCTA252.8077066E-670.0000153
GACATCT252.8077066E-670.0000149
GACAAAT252.8077066E-670.0000126
CGTAATC252.8077066E-670.0000148
CGTCATA150.002214429270.048
CAGCTAG301.0075382E-770.054
ACTGCAT150.002214429270.053
GTATGAA353.6325218E-970.042
CAAGAAA353.6325218E-970.057
AAACCAC401.3096724E-1070.03
AATCTGC150.002214429270.051
Produced by FastQC (version 0.11.5)