FastQCFastQC Report
Wed 25 May 2016
SRR1780810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454926
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6062713.32678281742525No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG9570.21036388335685363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC200.00694701952.5044519
CCGTTCC200.00694701952.5044520
GTCGACC200.006953062552.4929128
ATTTCGG305.876364E-446.66549348
CCGGCGA350.001254445940.0033921
GCCGTAT1850.039.72436554
ACAGTGT459.2004506E-538.8836366
CGGTGGT2350.038.7224346
TCGGTGG2350.038.7224345
CTCGGTG2350.038.71817444
TGGTCGC2100.038.3323749
TTAGGTG654.8624315E-737.69136449
ACGGGTT753.528112E-837.34060337
ATGACGG753.528112E-837.34060334
ATCTCGG2450.035.70946542
TGACGGG904.489266E-935.00681335
TACGATT400.002416895735.00296829
CGGGTTG806.238406E-834.99912338
TCGCCGT2300.034.99527752
CGCCGTA2200.034.99527453
Produced by FastQC (version 0.11.5)