FastQCFastQC Report
Wed 25 May 2016
SRR1780810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454926
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT318026.990587480161609No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG59741.3131806051973287No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT41170.9049823487776033No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC18990.41743052716265944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16960.37280788523847835No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11240.24707315035851987No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT9180.20179106052412918No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC7110.1562891547196687No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT5840.12837252652079678Illumina PCR Primer Index 3 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT5060.1112268808553479No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG4680.10287387399269332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA207.914602E-570.0158339
TAACCGA207.914602E-570.0158311
CGTACCT150.002223012770.0081341
GTACGTC150.002223012770.008139
AGGGGGG45750.064.415770
GACGTAT308.377132E-658.32728246
CGAATGC800.056.8816141
CGTCTTA1050.056.66079348
TGACGTA252.3907208E-455.9941945
GCCGTCT60100.053.2224446
CCGTCTT59600.053.08174547
TATGCCG62550.052.81046343
GTATGCC62600.052.60058642
TCTCGTA64350.052.5526838
CGTCTTC60700.052.52338848
TTACCCG200.00694917752.5003285
AGTCGAC200.00694917752.5003285
GCTCTAC200.00695219952.49455644
TGTCTTC200.00695522252.4887930
TCGTATG64950.052.4387440
Produced by FastQC (version 0.11.5)