FastQCFastQC Report
Wed 25 May 2016
SRR1780809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557766
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520349.32900176776641No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG6920.1240663647479409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG350.001254904840.0019955
CATCGCG350.001254904840.0019957
TCGTTGT400.002417776535.00174734
ATCGCGC501.7106574E-434.9954728
GTCGCCG1600.032.81119551
CGGCTGA552.9937652E-431.81406626
TCTCGGT1900.031.31454743
GACGGTT450.00430363731.11545666
ACGGTTT450.00430363731.11545667
TGGTCGC1700.030.88112649
CGGTGGT1850.030.2690746
TCGCCGT1650.029.70111752
CTCGGTG1900.029.47251544
TCGGTGG2150.029.30116345
CGTATCA1800.029.17073856
CGCCGTA1750.028.0039153
CATCGTT500.0072054828.00139832
ACATCGC500.0072054828.0013986
GTAGATC2050.027.31598938
GCCGTAT1800.027.22602354