FastQCFastQC Report
Wed 25 May 2016
SRR1780809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557766
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT262184.700537501389471No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG49490.8872896519328894No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT32990.5914666724038395No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC14680.2631928084537243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12390.2221361646281774No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT8410.15078007623268538No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT7080.12693495121610138No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC6530.11707418523179972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG39150.063.4756970
TGCCGAC1000.055.9966345
ATCGTAT2100.053.3444739
CCGTCTT49300.051.39649647
CGTCTTC49000.051.3494548
GCCGTCT49800.051.23185346
TATGCCG54200.051.20540243
TCGTATG55300.051.08661740
CGTATGC53800.051.0143741
CTCGTAT53700.050.84860639
TCTCGTA54050.050.7784138
GTATGCC54800.050.7724942
ATCTCGT53750.050.73617637
TGCCGTC53400.050.53066645
ATGCCGT54600.050.3815844
TATCGTA2250.049.78817438
GATCTCG55550.049.72235536
GGATCTC55650.049.69145635
GGGATCT57000.049.606634
TGGGATC56800.049.59644333