FastQCFastQC Report
Wed 25 May 2016
SRR1780808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101559
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4979549.030612747270055No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG7860.7739343632765191No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATC3940.38795183095540525No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1260.12406581396035801No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1120.11028072352031823No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGAC150.002216140570.0231552
TGACAGA150.002220476369.9886840
CTGCCGG150.002220476369.9886818
GATATGC150.002220476369.988688
GAACCTA150.002220476369.988686
TTTCCCC200.006924819752.51736521
CGGGGTC200.006924819752.51736555
ACGCGCA200.006924819752.51736552
GGATATG200.00693832952.491517
TGGTCGC1650.036.0547749
TCGCCGT1750.034.01124252
GTGGTCG1750.033.99449548
GTCGCCG1800.033.0664951
GCCGTAT1800.033.0664954
GGTCGCC1800.033.0502150
GGTGGTC1700.032.9520747
CGGTGGT1600.032.82335346
CGTATCA1850.032.172856
CCGTATC1850.032.172855
CATTAAA1850.032.1569661
Produced by FastQC (version 0.11.5)