FastQCFastQC Report
Wed 25 May 2016
SRR1780808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101559
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2509124.705836016502722No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG52905.208794887700745No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT35343.4797506867928982No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC14841.4612195866442166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13241.303675695900905No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT9630.9482172924113077No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT6940.6833466260991148No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC5090.5011865024271606No Hit
TCATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG4710.46376982837562397No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT4470.4401382447641273TruSeq Adapter, Index 10 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4300.4233992063726504No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTA3770.37121279256392836No Hit
CATAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3520.3465965596352859No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT2410.23730048543211335No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT2350.23139258952923916No Hit
CACTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1860.18314477298909992No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC1540.15163599484043758No Hit
CTAAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1530.15065134552329187No Hit
CTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT1470.14474344962041769No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1430.1408048523518349No Hit
CTTATAAACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1420.1398202030346892No Hit
CTTATACAAATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1390.1368662550832521No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT1390.1368662550832521No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGGCTAATCTCGTATGCCGT1290.12701976191179512No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG1270.12505046327750372No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1180.11618861942319243No Hit
CAAAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1060.10437282761744404No Hit
TCTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG1050.10338817830029835No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT1050.10338817830029835No Hit
CTTATACACATCTCCGAGACCACGAGACACCGGCTAATCTCGTATGCCGT1030.10141887966600696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTA207.880706E-570.0236426
CTGAATG150.002216079370.0236454
CTCGTAA550.069.9891739
ACGCAAA150.002220414869.9891711
TCCTGGC150.002220414869.9891712
CGTCTAC600.069.9891748
AAGCCGT401.3278623E-1069.9891744
TCTACGC150.002220414869.9891711
CTACGCG150.002220414869.9891712
CAGTGTG150.002220414869.989174
TCGTAAG600.069.9891740
GTAAGCA207.899971E-569.9891742
GACGACT150.002220414869.9891746
CCAGTGT150.002220414869.989173
CGTCAAC150.002220414869.9891748
TCGAAAG150.002220414869.9891740
TCTATGT150.002220414869.9891730
AGGGGGG37350.066.8035870
CGTAAGC750.065.3232341
TAAGCCG550.063.62651443
Produced by FastQC (version 0.11.5)