##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780808_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101559 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.949221634714796 32.0 32.0 32.0 27.0 32.0 2 28.14441851534576 32.0 27.0 32.0 13.0 32.0 3 29.18462174696482 32.0 32.0 32.0 22.0 32.0 4 29.641095323900394 32.0 27.0 32.0 27.0 32.0 5 28.986904164081963 32.0 27.0 32.0 22.0 32.0 6 33.55734105298398 37.0 32.0 37.0 27.0 37.0 7 33.07566045352947 37.0 32.0 37.0 22.0 37.0 8 32.153034196870784 37.0 32.0 37.0 13.0 37.0 9 32.588416585433094 37.0 32.0 37.0 22.0 37.0 10-11 33.68067822644965 37.0 34.5 37.0 24.5 37.0 12-13 33.85582764698353 37.0 37.0 37.0 24.5 37.0 14-15 33.51571500310165 37.0 32.0 37.0 22.0 37.0 16-17 31.974891442412783 37.0 29.5 37.0 17.5 37.0 18-19 33.02591104678069 37.0 32.0 37.0 22.0 37.0 20-21 33.366796640376535 37.0 34.5 37.0 22.0 37.0 22-23 32.91747654073002 37.0 34.5 37.0 17.5 37.0 24-25 33.41982492935141 37.0 32.0 37.0 22.0 37.0 26-27 33.491236621077405 37.0 34.5 37.0 24.5 37.0 28-29 33.415694325465985 37.0 34.5 37.0 20.0 37.0 30-31 33.5148337419628 37.0 34.5 37.0 24.5 37.0 32-33 33.043821817859566 37.0 32.0 37.0 13.0 37.0 34-35 33.80422217627192 37.0 37.0 37.0 24.5 37.0 36-37 32.4541891905198 37.0 32.0 37.0 17.5 37.0 38-39 31.527008930769306 37.0 29.5 37.0 13.0 37.0 40-41 32.955533236837695 37.0 32.0 37.0 17.5 37.0 42-43 33.504933093078904 37.0 37.0 37.0 22.0 37.0 44-45 33.3207199755807 37.0 32.0 37.0 22.0 37.0 46-47 32.93989208243484 37.0 32.0 37.0 17.5 37.0 48-49 32.171712994417035 37.0 32.0 37.0 13.0 37.0 50-51 32.80810661782806 37.0 32.0 37.0 17.5 37.0 52-53 32.43411711418978 37.0 32.0 37.0 13.0 37.0 54-55 32.282505735582276 37.0 32.0 37.0 13.0 37.0 56-57 32.12793548577674 37.0 32.0 37.0 13.0 37.0 58-59 32.171658838704595 37.0 32.0 37.0 13.0 37.0 60-61 32.371877430853004 37.0 32.0 37.0 13.0 37.0 62-63 32.99704605204856 37.0 32.0 37.0 22.0 37.0 64-65 33.05220610679507 37.0 32.0 37.0 17.5 37.0 66-67 32.776533837473785 37.0 32.0 37.0 13.0 37.0 68-69 31.764732815407793 37.0 29.5 37.0 13.0 37.0 70-71 28.57327760218198 34.5 17.5 37.0 13.0 37.0 72-73 30.95472582439764 37.0 29.5 37.0 13.0 37.0 74-75 32.48190214555086 37.0 32.0 37.0 13.0 37.0 76 32.77056686261188 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.28983752458266565 11101 2 -1.309706185229384 11101 3 0.6096771445239604 11101 4 -0.7284524087718935 11101 5 -0.772824637533482 11101 6 -0.6647047065648977 11101 7 -0.9303541236384376 11101 8 -0.7456489759282228 11101 9 0.2951886840309115 11101 10-11 0.5344907310389502 11101 12-13 0.24560659974027033 11101 14-15 0.9977439920899229 11101 16-17 -0.08257749701392214 11101 18-19 0.43966672464904377 11101 20-21 -0.49395187016097 11101 22-23 1.0433372898756659 11101 24-25 -0.036288612012604915 11101 26-27 -0.0055672255030501105 11101 28-29 -0.7569213580064158 11101 30-31 -0.6066901551251576 11101 32-33 0.7680153544997594 11101 34-35 0.20558314770884323 11101 36-37 -0.3505507895940596 11101 38-39 -0.7404038253085439 11101 40-41 -0.0014432108724165005 11101 42-43 -0.4064674426728132 11101 44-45 0.1837366315922253 11101 46-47 -0.4882322109707573 11101 48-49 0.10205662876202126 11101 50-51 0.3784329152864956 11101 52-53 -0.13490522676666927 11101 54-55 -0.9546681826982848 11101 56-57 -0.6768023038751494 11101 58-59 0.23217613366058032 11101 60-61 0.1828977167001895 11101 62-63 -0.5804244236877594 11101 64-65 -1.0103023990271822 11101 66-67 -1.4850510758825628 11101 68-69 -1.9941887351687306 11101 70-71 1.6294403501944146 11101 72-73 0.6638167010738592 11101 74-75 -0.20965111517475776 11101 76 1.1206760881929227 11102 1 -1.4131736458884383 11102 2 -0.7725522269972309 11102 3 -0.2866317432856569 11102 4 0.0950055805433081 11102 5 0.06253233186913931 11102 6 0.20416367614514996 11102 7 -0.7834381450080343 11102 8 -3.757062283359012 11102 9 -0.9299205923168294 11102 10-11 -0.21368799649869175 11102 12-13 0.24548518157610744 11102 14-15 0.8639411751885149 11102 16-17 -0.14182956112271583 11102 18-19 -0.09299476150928854 11102 20-21 0.5578936859342178 11102 22-23 -0.4274009325624064 11102 24-25 -0.978129311381231 11102 26-27 -0.5297294401703638 11102 28-29 0.3008736881324836 11102 30-31 -0.025097148811411785 11102 32-33 0.20657776343613676 11102 34-35 0.964568091856485 11102 36-37 0.068827549405448 11102 38-39 -0.5420065450754201 11102 40-41 -0.6702144590511452 11102 42-43 -0.33920177972964183 11102 44-45 -0.5000904689420196 11102 46-47 -1.1994998166045612 11102 48-49 0.07352336018504957 11102 50-51 -0.6405083183220484 11102 52-53 -0.4036036240469052 11102 54-55 -1.2790975173267434 11102 56-57 -0.9348159027095342 11102 58-59 -0.9412090047561215 11102 60-61 0.412985137778378 11102 62-63 -0.027728940443466854 11102 64-65 0.8356179506571308 11102 66-67 -0.50350663683448 11102 68-69 0.39580640810470413 11102 70-71 0.5721309767121454 11102 72-73 -1.383414964783352 11102 74-75 0.4191735569961992 11102 76 1.2962467535644606 11103 1 -0.1548886417459343 11103 2 -1.5242092278257324 11103 3 0.14770959888501523 11103 4 -0.5387972363581 11103 5 -1.3914858951399722 11103 6 0.24807419810041154 11103 7 -0.1539849552814374 11103 8 0.343600516972387 11103 9 -0.1525800786465723 11103 10-11 -0.4708338125715983 11103 12-13 -1.0010972542151109 11103 14-15 -0.6125501255571351 11103 16-17 0.025941772762951842 11103 18-19 0.841035893006044 11103 20-21 -0.8499356429804408 11103 22-23 -0.7436809657024241 11103 24-25 -1.571024920329041 11103 26-27 0.5768364256125622 11103 28-29 0.3064660659286744 11103 30-31 0.9430055852399519 11103 32-33 -0.13031121750835695 11103 34-35 0.11608010511249489 11103 36-37 -1.3530862865514663 11103 38-39 -0.24395352104891188 11103 40-41 -0.5227413853146032 11103 42-43 -0.703120586689046 11103 44-45 -0.6420747274341494 11103 46-47 0.2764736713821847 11103 48-49 0.957533302190015 11103 50-51 1.0396905217773735 11103 52-53 0.38740714645847873 11103 54-55 -0.7650130102844912 11103 56-57 -1.1210213708122652 11103 58-59 -0.6418096675564513 11103 60-61 -1.0191813908048815 11103 62-63 -0.08448121881960446 11103 64-65 0.8416245786604506 11103 66-67 -0.62374275945254 11103 68-69 -0.03729218344459184 11103 70-71 0.2808095185514148 11103 72-73 0.2033704536093559 11103 74-75 0.815196755007797 11103 76 0.08974302531260037 11104 1 -0.4494519216033339 11104 2 -1.8410541476118283 11104 3 0.7319341978154945 11104 4 -0.4933426909035532 11104 5 -0.722145431681863 11104 6 0.1027979057652999 11104 7 -0.1571043848714595 11104 8 -0.2205706060222674 11104 9 0.6018762493391634 11104 10-11 -0.15777677157338132 11104 12-13 -0.11228263745931599 11104 14-15 0.103804598150532 11104 16-17 -0.2931313110160154 11104 18-19 0.31897706947663096 11104 20-21 1.180813020121164 11104 22-23 0.13555254588403187 11104 24-25 -0.5088591449279747 11104 26-27 0.00932305662860955 11104 28-29 -0.25012929949021867 11104 30-31 -0.014213666096942745 11104 32-33 0.5548492102991318 11104 34-35 -0.3484920874008779 11104 36-37 -1.216277017389256 11104 38-39 -0.585753877555149 11104 40-41 -0.8614223122308289 11104 42-43 0.5459881477138069 11104 44-45 0.5364012083947216 11104 46-47 0.5117677890292427 11104 48-49 0.7783889171632765 11104 50-51 -0.05077293633314639 11104 52-53 -0.8256053312598794 11104 54-55 -1.4619827072541867 11104 56-57 0.31614440458880466 11104 58-59 -0.2654727691607306 11104 60-61 -1.7154826385767166 11104 62-63 -1.3852833578570412 11104 64-65 0.5100737765214163 11104 66-67 0.5854818394779358 11104 68-69 -1.029716425868834 11104 70-71 0.04662948290079072 11104 72-73 0.8388428243758419 11104 74-75 0.5108295535817717 11104 76 1.431543381818841 11105 1 -0.4119123467503272 11105 2 -0.6504164271233606 11105 3 -0.8033439568656462 11105 4 -0.9417823109849657 11105 5 -0.5916614877650836 11105 6 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66-67 0.6621439833042615 11105 68-69 -1.8022175565789205 11105 70-71 -0.25990602139590635 11105 72-73 0.5723344571212117 11105 74-75 -0.062327388978154374 11105 76 -1.6299672468559692 11106 1 -0.17158854204503982 11106 2 -1.237818400308285 11106 3 -0.35204114887171656 11106 4 0.2917112382181699 11106 5 -0.3526025451898249 11106 6 -2.3730623921288974 11106 7 0.37268503474847137 11106 8 0.6900611351179506 11106 9 -1.6682829699725943 11106 10-11 -0.24663141975005942 11106 12-13 -0.1709626580037451 11106 14-15 -0.858188211299904 11106 16-17 0.46213319848578394 11106 18-19 0.2977906287986656 11106 20-21 -0.4324381355028777 11106 22-23 -0.4872004073774008 11106 24-25 -1.2962243221236562 11106 26-27 -1.377425787747356 11106 28-29 -0.7665648413494921 11106 30-31 0.014291726815230277 11106 32-33 -0.6516222843079547 11106 34-35 -0.7691392368615837 11106 36-37 -1.299995558734917 11106 38-39 0.5185090911145984 11106 40-41 -0.39711825470643447 11106 42-43 0.1864606695422637 11106 44-45 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0.32797111843468585 11108 6 -0.27319277022537847 11108 7 -1.989505317272389 11108 8 -0.023367808819465097 11108 9 -0.8419465944836766 11108 10-11 -0.09787001166661469 11108 12-13 -0.10121037638705843 11108 14-15 0.3746670690130003 11108 16-17 0.7476612297765293 11108 18-19 0.8920539925535564 11108 20-21 -0.1954107561026177 11108 22-23 0.6954593057907914 11108 24-25 -0.33403397010279434 11108 26-27 -1.2759916286860786 11108 28-29 -1.2258868752477952 11108 30-31 -0.3379898898731639 11108 32-33 0.22477928022919968 11108 34-35 -0.44149908040787267 11108 36-37 -0.8908690919813296 11108 38-39 0.8969967051954306 11108 40-41 -0.6462707970793105 11108 42-43 0.49936810109375784 11108 44-45 0.048988620982143516 11108 46-47 -0.8266937494322946 11108 48-49 -0.7768558380814738 11108 50-51 -0.07116920672941518 11108 52-53 -2.0639503196048707 11108 54-55 -0.18039762566910866 11108 56-57 -0.03979965135525276 11108 58-59 -1.5609273146152773 11108 60-61 -1.3066248297189098 11108 62-63 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11109 42-43 1.1993681010937607 11109 44-45 -0.3817806097870857 11109 46-47 -0.9190014417399865 11109 48-49 -0.1537789150045512 11109 50-51 -1.7634768990371086 11109 52-53 -0.2639503196048665 11109 54-55 0.41960237433089276 11109 56-57 0.1448157332601312 11109 58-59 -1.3224657761537415 11109 60-61 0.3164520933580093 11109 62-63 -1.1310875536612386 11109 64-65 -0.8979681815205467 11109 66-67 -1.2935391395430393 11109 68-69 1.1127078165554138 11109 70-71 -0.875864689593385 11109 72-73 0.1520129875460121 11109 74-75 0.11700670975893246 11109 76 -0.1204379701625129 11110 1 0.8525848394498219 11110 2 1.685771485771184 11110 3 -1.0989152805749676 11110 4 -0.17814149865318285 11110 5 -2.127262172864178 11110 6 -0.17911963025059663 11110 7 1.7522349097137422 11110 8 -1.9895720963352304 11110 9 -1.1070159513563134 11110 10-11 -0.29761780485703326 11110 12-13 0.18441383546667112 11110 14-15 -1.7809445325002429 11110 16-17 -1.1741546592524763 11110 18-19 -0.6277442419987835 11110 20-21 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0.44048323323545446 21611 76 -1.0403180900426356 21612 1 0.7758445210844123 21612 2 2.540368777289359 21612 3 -0.0012673832121734563 21612 4 1.0409129085694353 21612 5 1.1072353324815083 21612 6 1.063930975594019 21612 7 1.10837651104422 21612 8 2.033711343911861 21612 9 0.6387668949254675 21612 10-11 0.6439360084002743 21612 12-13 1.2549769145828407 21612 14-15 1.1209323978870245 21612 16-17 1.4176723780485467 21612 18-19 1.1631799680273573 21612 20-21 -0.48782993113048434 21612 22-23 0.8937870649881106 21612 24-25 0.8014175349139308 21612 26-27 0.7286906455614215 21612 28-29 1.1306348638826407 21612 30-31 1.067472763415573 21612 32-33 0.6253366938300857 21612 34-35 0.8201508638507704 21612 36-37 0.262642613704287 21612 38-39 1.006472736410597 21612 40-41 1.3638741304569209 21612 42-43 1.40237813453858 21612 44-45 1.0660454771694248 21612 46-47 1.1639417020727194 21612 48-49 1.7823414863332445 21612 50-51 1.6228107263809548 21612 52-53 2.043630505367261 21612 54-55 1.2349869897155088 21612 56-57 2.372797896024899 21612 58-59 1.7506668882832699 21612 60-61 2.375426452332377 21612 62-63 1.0847429926040917 21612 64-65 -0.15560474785277023 21612 66-67 1.2798164903343263 21612 68-69 1.219954193367002 21612 70-71 1.4797651877756266 21612 72-73 1.1566952617935051 21612 74-75 0.4498941122115454 21612 76 1.0639544490125132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 5.0 17 15.0 18 21.0 19 59.0 20 140.0 21 306.0 22 597.0 23 894.0 24 1345.0 25 1761.0 26 2297.0 27 3092.0 28 3903.0 29 4849.0 30 6338.0 31 8101.0 32 10948.0 33 15949.0 34 20730.0 35 17344.0 36 2865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.230259334771347 11.218469599818771 14.770164189541903 55.781106875867984 2 10.124164278892072 20.406857097844604 48.327573134828036 21.141405488435293 3 9.99911381561457 19.015547612717732 56.17522819247925 14.810110379188451 4 6.818696521233962 52.39909806122549 27.097549207849625 13.684656209690917 5 6.863990389822665 30.333106863990388 54.778010811449505 8.024891934737443 6 13.897039998424484 61.85576146680584 16.953541957973098 7.293656576796581 7 12.868888779479102 24.332627640194968 12.935847570282114 49.86263601004382 8 11.455410155673057 61.73652753571815 9.996159867663131 16.81190244094566 9 11.44469170326316 18.558853069182142 9.875145237204357 60.12130999035035 10-11 11.88138797534414 38.89403099706571 33.18793201914177 16.036649008448375 12-13 12.093685070849704 13.45799730190147 38.00577038590687 36.44254724134196 14-15 11.72717336720527 12.396734902864345 15.683001998838114 60.19308973109227 16-17 37.15979361140651 33.255543739414705 10.60990980345819 18.974752845720587 18-19 38.42003170570801 18.53159247334062 11.169861853700805 31.878513967250566 20-21 13.111057500578502 12.953508180256115 10.490815366966832 63.44461895219855 22-23 11.98521049444409 38.24101379992812 10.650511774002176 39.123263931625615 24-25 37.32411701572485 35.014129717701046 10.947331108025876 16.714422158548235 26-27 38.49312438148974 39.476832636663076 10.800112254996087 11.2299307268511 28-29 13.303694075594876 39.308450142534305 10.888734620327016 36.499121161543805 30-31 12.060477161059088 18.530115499364904 10.86511289004421 58.5442944495318 32-33 58.204627026601216 12.348912663518963 11.414940598982822 18.031519710896998 34-35 19.29518801878711 12.882659340875746 32.4944121151252 35.32774052521195 36-37 11.133922153624987 58.188836046042205 17.90240155968452 12.774840240648293 38-39 11.88845617956507 20.562445535243928 36.62985628689449 30.919241998296513 40-41 11.31379673667937 11.978474294210905 38.97669197364923 37.7310369954605 42-43 36.78413880026783 11.938240970499034 34.877801410059476 16.39981881917366 44-45 12.615761824815003 36.79121268665902 39.166268690518976 11.426756798007002 46-47 36.09320919882036 13.629851363526708 18.597037117269696 31.679902320383235 48-49 33.5351864433482 11.503657972213196 13.169684616823718 41.791470967614885 50-51 15.894029953622105 12.308358852664021 37.84032612227616 33.95728507143772 52-53 10.233661884458973 12.698779995470524 60.02983546185886 17.037722658211646 54-55 10.32425140561458 12.677609618243944 40.789901237728564 36.20823773841291 56-57 35.078485051404265 11.663942175129003 18.884173001930122 34.37339977153661 58-59 11.927034611786716 13.536999655359164 57.6845059327458 16.851459800108316 60-61 35.07535065996446 33.93905975373799 19.302175593377218 11.683413992920338 62-63 11.488452468281828 65.55138171595681 11.158100995977687 11.802064819783672 64-65 9.769764052467798 67.83983928782447 11.110509315791548 11.279887343916178 66-67 10.18053989651081 68.10346949727492 10.211064776749888 11.504925829464387 68-69 10.761004957831355 67.62836450645699 9.972773776173854 11.637856759537794 70-71 29.59529319088179 48.31815272512432 9.98178327014918 12.104770813844715 72-73 64.09557053323947 13.620228735162494 9.83964906925565 12.444551662342395 74-75 66.94581887108288 11.543706767102382 9.50052925682495 12.009945104989784 76 67.12030645304237 10.960226097745917 9.846478054929147 12.072989394282562 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.5 14 2.5 15 8.0 16 13.0 17 13.0 18 20.5 19 27.5 20 43.5 21 60.0 22 74.5 23 92.5 24 98.5 25 101.0 26 129.0 27 209.5 28 284.5 29 307.0 30 356.5 31 449.0 32 615.0 33 738.0 34 807.0 35 1023.5 36 1242.5 37 1314.0 38 1509.0 39 1884.5 40 2196.5 41 2563.5 42 2799.0 43 2986.0 44 3388.0 45 4193.0 46 4783.0 47 6013.0 48 12034.5 49 24911.0 50 32996.0 51 18401.0 52 3012.5 53 1896.0 54 1573.0 55 1524.0 56 1321.0 57 1095.0 58 1023.0 59 943.0 60 791.5 61 647.0 62 574.0 63 529.5 64 485.0 65 429.0 66 351.5 67 330.0 68 311.5 69 255.5 70 215.0 71 212.0 72 179.0 73 125.5 74 98.5 75 92.0 76 82.0 77 61.5 78 48.5 79 46.0 80 40.5 81 33.5 82 29.0 83 26.0 84 23.5 85 13.0 86 6.0 87 7.0 88 8.5 89 6.0 90 2.5 91 3.5 92 4.0 93 2.5 94 3.0 95 5.5 96 6.0 97 16.0 98 56.0 99 685.5 100 1285.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02953947951437096 2 0.0 3 0.0 4 0.0 5 0.0 6 0.004923246585728493 7 0.003938597268582794 8 0.0 9 9.846493171456986E-4 10-11 9.846493171456986E-4 12-13 0.005907895902874191 14-15 0.0 16-17 0.0029539479514370955 18-19 0.0 20-21 0.0034462726100099447 22-23 4.923246585728493E-4 24-25 0.0 26-27 0.004430921927155644 28-29 0.004430921927155644 30-31 0.0 32-33 0.0034462726100099447 34-35 0.0 36-37 0.0 38-39 0.0024616232928642463 40-41 0.005907895902874191 42-43 0.0029539479514370955 44-45 0.0034462726100099447 46-47 0.0024616232928642463 48-49 0.0 50-51 0.001969298634291397 52-53 0.001969298634291397 54-55 0.001969298634291397 56-57 0.010831142488602685 58-59 0.003938597268582794 60-61 4.923246585728493E-4 62-63 4.923246585728493E-4 64-65 0.010831142488602685 66-67 0.0024616232928642463 68-69 0.0024616232928642463 70-71 0.003938597268582794 72-73 4.923246585728493E-4 74-75 0.0014769739757185478 76 0.009846493171456985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 101559.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.464114455636626 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06313685204209 27.68440020086846 2 10.16186800677458 7.2076330015065135 3 5.042063470028042 5.364369479809765 4 2.562678735041786 3.6353252789019193 5 1.5659272010439516 2.77671107435087 6 0.7302107338201405 1.5537766224559122 7 0.4914346003276231 1.2199805039435205 8 0.31096426687397616 0.8822457881625458 9 0.15825860010550571 0.5051250996957434 >10 0.749646000499764 4.417136836715604 >50 0.08051753338701169 1.982099075414291 >100 0.061082266707388186 4.479169743695783 >500 0.008329400005552934 2.132750420937583 >1k 0.008329400005552934 6.244645969338021 >5k 0.0027764666685176445 5.208794887700745 >10k+ 0.0027764666685176445 24.705836016502722 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 25091 24.705836016502722 No Hit TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG 5290 5.208794887700745 No Hit CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 3534 3.4797506867928982 No Hit CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC 1484 1.4612195866442166 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1324 1.303675695900905 No Hit CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 963 0.9482172924113077 No Hit CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 694 0.6833466260991148 No Hit TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC 509 0.5011865024271606 No Hit TCATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG 471 0.46376982837562397 No Hit ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT 447 0.4401382447641273 TruSeq Adapter, Index 10 (95% over 21bp) CAAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 430 0.4233992063726504 No Hit CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTA 377 0.37121279256392836 No Hit CATAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 352 0.3465965596352859 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT 241 0.23730048543211335 No Hit CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT 235 0.23139258952923916 No Hit CACTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC 186 0.18314477298909992 No Hit CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC 154 0.15163599484043758 No Hit CTAAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 153 0.15065134552329187 No Hit CTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT 147 0.14474344962041769 No Hit CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC 143 0.1408048523518349 No Hit CTTATAAACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 142 0.1398202030346892 No Hit CTTATACAAATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 139 0.1368662550832521 No Hit CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT 139 0.1368662550832521 No Hit CTTATACACATCTCCGAGCCCACGAGACAACGGCTAATCTCGTATGCCGT 129 0.12701976191179512 No Hit CCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG 127 0.12505046327750372 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC 118 0.11618861942319243 No Hit CAAAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT 106 0.10437282761744404 No Hit TCTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG 105 0.10338817830029835 No Hit CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT 105 0.10338817830029835 No Hit CTTATACACATCTCCGAGACCACGAGACACCGGCTAATCTCGTATGCCGT 103 0.10141887966600696 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5011865024271606 0.0 2 0.0 0.0 0.0 0.5051250996957434 0.0 3 0.0 0.0 0.0 0.5100483462814719 0.0 4 0.0 0.0 0.0 0.5720812532616508 0.0 5 0.0 0.0 0.0 0.5888202916531278 0.0 6 0.0 0.0 0.0 0.6055593300446046 0.0 7 0.0 0.0 0.0 0.6055593300446046 0.0 8 0.0 0.0 0.0 0.607528628678896 0.0 9 0.0 0.0 0.0 0.6134365245817702 0.0 10 0.0 0.0 0.0 0.6134365245817702 0.0 11 0.0 0.0 0.0 0.6222983684360814 0.0 12 0.0 0.0 0.0 0.6439606534132869 0.0 13 0.0 0.0 0.0 0.6508531986333067 0.0 14 0.0 0.0 0.0 0.666607587707638 0.0 15 0.0 0.0 0.0 0.6695615356590751 0.0 16 0.0 0.0 0.0 0.6715308342933665 0.0 17 0.0 0.0 0.0 0.6725154836105122 0.0 18 0.0 0.0 0.0 0.6744847822448035 0.0 19 0.0 0.0 0.0 0.6813773274648234 0.0 20 0.0 0.0 0.0 0.6813773274648234 0.0 21 0.0 0.0 0.0 0.6823619767819691 0.0 22 0.0 0.0 0.0 0.6872852233676976 0.0 23 0.0 0.0 0.0 0.6971317165391546 0.0 24 0.0 0.0 0.0 0.699101015173446 0.0 25 0.0 0.0 0.0 0.7069782097106115 0.0 26 0.0 0.0 0.0 0.7069782097106115 0.0 27 0.0 0.0 0.0 0.7128861056134858 0.0 28 0.0 0.0 0.0 0.7286404946878169 0.0 29 0.0 0.0 0.0 0.7522720782993138 0.0 30 0.0 0.0 0.0 0.7887041030337045 0.0 31 0.0 0.0 0.0 0.8399058675252808 0.0 32 0.0 0.0 0.0 0.8871690347482744 0.0 33 0.0 0.0 0.0 0.9442786951427249 0.0 34 0.0 0.0 0.0 1.038805029588712 0.0 35 0.0 0.0 0.0 1.1638554928662157 0.0 36 0.0 0.0 0.0 1.368662550832521 0.0 37 0.0 0.0 0.0 1.6935968254906015 0.0 38 0.0 0.0 0.0 1.9624060890713773 0.0 39 0.0 0.0 0.0 2.285371065095166 0.0 40 0.0 0.0 0.0 2.6930158823934858 0.0 41 0.0 0.0 0.0 3.0760444667631623 0.0 42 0.0 0.0 0.0 3.492551127915793 0.0 43 0.0 0.0 0.0 3.9415512165342315 0.0 44 0.0 0.0 0.0 4.391535954469815 0.0 45 0.0 0.0 0.0 4.773579889522346 0.0 46 0.0 0.0 0.0 5.195009797260705 0.0 47 0.0 0.0 0.0 5.58788487480184 0.0 48 0.0 0.0 0.0 5.965005563268642 0.0 49 0.0 0.0 0.0 6.32243326539253 0.0 50 0.0 0.0 0.0 6.65918333185636 0.0 51 0.0 0.0 0.0 7.0648588505203875 0.0 52 0.0 0.0 0.0 7.40751681288709 0.0 53 0.0 0.0 0.0 7.786606799988184 0.0 54 0.0 0.0 0.0 8.13320335962347 0.0 55 0.0 0.0 0.0 8.528047735798895 0.0 56 0.0 0.0 0.0 8.977047824417333 0.0 57 0.0 0.0 0.0 9.26653472365817 0.0 58 0.0 0.0 0.0 9.578668557193355 0.0 59 0.0 0.0 0.0 9.884894494825668 0.0 60 0.0 0.0 0.0 10.146811213186425 0.0 61 0.0 0.0 0.0 10.418574424718637 0.0 62 0.0 0.0 0.0 10.66473675400506 0.0 63 0.0 0.0 0.0 10.907945135340048 0.0 64 0.0 0.0 0.0 11.19152413867801 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGTA 20 7.880706E-5 70.02364 26 CTGAATG 15 0.0022160793 70.02364 54 CTCGTAA 55 0.0 69.98917 39 ACGCAAA 15 0.0022204148 69.98917 11 TCCTGGC 15 0.0022204148 69.98917 12 CGTCTAC 60 0.0 69.98917 48 AAGCCGT 40 1.3278623E-10 69.98917 44 TCTACGC 15 0.0022204148 69.98917 11 CTACGCG 15 0.0022204148 69.98917 12 CAGTGTG 15 0.0022204148 69.98917 4 TCGTAAG 60 0.0 69.98917 40 GTAAGCA 20 7.899971E-5 69.98917 42 GACGACT 15 0.0022204148 69.98917 46 CCAGTGT 15 0.0022204148 69.98917 3 CGTCAAC 15 0.0022204148 69.98917 48 TCGAAAG 15 0.0022204148 69.98917 40 TCTATGT 15 0.0022204148 69.98917 30 AGGGGGG 3735 0.0 66.80358 70 CGTAAGC 75 0.0 65.32323 41 TAAGCCG 55 0.0 63.626514 43 >>END_MODULE