FastQCFastQC Report
Wed 25 May 2016
SRR1780807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69963
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5097672.86136958106427No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG7711.1020110629904378No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1230.17580721238368852No Hit
TCTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGT950.13578605834512528No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780.1114875005359976No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780.1114875005359976No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750.10719951974615154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTG150.002213366870.0221834
AGTGTGG150.002213366870.0221835
GGAGTGT150.002213366870.0221833
GTCTGGC150.00221965969.9721210
GCGTCTG207.892315E-569.972128
GTGGCGC207.892315E-569.9721212
TGGCGCT252.8198428E-669.9721213
CGTCTGG207.892315E-569.972129
CCGGCGG150.00221965969.9721221
AAAGGGG252.3801727E-455.977770
TGGTCGC1800.054.46169349
GTCGCCG1800.054.46169351
GGTCGCC1800.054.46169350
TCGGTGG1750.054.01710545
TCTCGGT1750.054.01710543
CTCGGTG1750.054.01710544
TCGCCGT1700.053.54636852
GTATCAT1650.053.04710457
CCGTATC1650.053.04710455
CGTATCA1650.053.04710456