FastQCFastQC Report
Wed 25 May 2016
SRR1780807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69963
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2572036.76228863828023No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG48256.896502437002415No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT32634.663893772422567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13981.9981990480682648No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC13451.922444720780984No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT8041.1491788516787445No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT6470.9247745236768006No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC5560.7947057730514702No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT3980.5688721181195775RNA PCR Primer, Index 14 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3650.5217043294312708No Hit
CAAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT3430.49025913697239965No Hit
CTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT3030.4330860597744522No Hit
CATAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2830.4044995211754785No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT2670.38163029029629947No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT2480.3544730786272744No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT2310.33017452081814674No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1790.255849520460815No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT1640.2344096165115847No Hit
CACTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC1580.22583365493189256No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT1550.2215456741420465No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1550.2215456741420465No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1440.20582307791261095No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT1410.2015350971227649No Hit
CTTATACAAATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1210.17294855852379115No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT1140.16294327001415035No Hit
TCTTATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCG1090.15579663536440688No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1050.15007932764461215No Hit
CTTATAAACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1010.1443620199248174No Hit
CTAAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1000.14293269299486872No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC950.13578605834512528No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT940.1343567314151766No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA900.12863942369538184No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC850.12149278904563841No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT820.11720480825579235No Hit
CAAAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT820.11720480825579235No Hit
CATATACACATCTCCGCGCCCACGAGACCGGTTCCCATCTCGTATGCCGT800.11434615439589496No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTAAGCCGT790.11291682746594628No Hit
TCCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC760.10862884667610022No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG740.10577019281620284No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGTTCCCATCTCGTATGCCGT720.10291153895630548No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGGTTCCCATCTCGTATGCCGT710.10148221202635678No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACG700.1000528850964081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAA353.6379788E-970.0178857
ACACGAG550.070.0178820
GATACCA252.8088944E-670.0178831
CACGTAA252.8088944E-670.0178839
GGATACC252.8088944E-670.0178830
GAGCACA550.070.0178816
CCGTAAT252.8088944E-670.0178847
TGCTTGC252.8088944E-670.0178855
GCTTGCA252.8088944E-670.0178856
CGAGCAC455.456968E-1270.0178815
CTTGCAA252.8088944E-670.0178857
ATACCAT252.8088944E-670.0178832
GCACACG550.070.0178818
GCCCTCT252.8088944E-670.0178846
ACACGTA150.002213905670.01787638
ACTGCAT301.0084841E-770.01787653
CAAGAGA150.002213905670.01787621
ACGTCTA207.866765E-570.01787647
CCTGTTC150.002213905670.01787628
ATGACGA207.866765E-570.01787644
Produced by FastQC (version 0.11.5)