FastQCFastQC Report
Wed 25 May 2016
SRR1780806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258678
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4638317.930786537703245No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG6010.23233518119051486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC1450.055.5240
CTCGGTG1450.055.5244
TGGTCGC1450.055.5249
TGTGCGC200.0069446152.5026133
AACGGGG200.0069446152.50261348
CGGTGGT1600.052.50261346
GAACGGG200.0069446152.50261347
GCCGTAT1400.052.50261354
CGCGTCT200.006949922552.4924667
TCGGTGG1550.051.93806545
TCTCGGT1500.051.33588443
CGTATCA1500.049.00243856
TCGCCGT1550.047.4217152
ATCTCGG1700.047.35529342
GTAGATC1800.046.6780138
ACGTTGG305.875067E-446.6599669
CGTTGGG305.875067E-446.65996610
GTCGCCG1750.046.00228551
TAGATCT1750.046.00228539
GATCTCG1750.046.00228541
Produced by FastQC (version 0.11.5)