FastQCFastQC Report
Wed 25 May 2016
SRR1780806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258678
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT70372.720370499230704No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT20460.7909447266485746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11790.4557789993737388No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG9310.3599069112951237No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT6040.23349492419146584No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT6020.23272176219083185No Hit
CATATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT5810.2246035611841749No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT4570.17666751714486736No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT4400.17009564013947842No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG3930.1519263331245796No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT3730.14419471311823967No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT3060.1182937860970009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT301.0209442E-770.0019447
GGCAACG150.002222705970.0019434
CGACACT150.002222705970.0019423
TGCGGAA301.0209442E-770.0019446
ATCAAGG207.9159385E-570.0019437
TGCCGAC401.3460522E-1070.0019445
ATTGCCG252.834302E-670.0019344
AATTCGC252.8375598E-669.988427
CTTTATA650.064.6046752
AGGGGGG19700.061.82911770
ATCGTAT401.0626536E-861.25169439
CCGTCAA352.9663352E-760.0016647
ATTCGCG352.9703006E-759.9900628
TTCGAGG700.059.9900629
ATCCGAA308.360445E-658.3349545
AATCTCG308.360445E-658.3349536
TTGCGGA308.360445E-658.3349545
TACGTAT308.360445E-658.3349546
TACGCGG601.8189894E-1258.3236729
CGAATGC601.8189894E-1258.3236741
Produced by FastQC (version 0.11.5)