FastQCFastQC Report
Wed 25 May 2016
SRR1780805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147256
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4957733.66721899277449No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG7060.47943717064160374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC150.002219076770.01562516
GTTGTGC150.002219076770.0156251
ACGGTAC150.00222206969.9918550
CGGTGGT1450.065.16482546
CTCGGTG1350.064.8292844
TCTCGGT1300.064.6298143
ATCTCGG1250.064.41437542
GCGTGTA1200.064.15919534
GTCGCCG1500.062.9926751
CCGTATC1300.061.936955
GATCTCG1300.061.936941
TGGTCGC1450.060.337849
GTATCAT1350.059.64293757
GCCGTAT1350.059.64293754
TGCGTGT1300.059.22387333
TCGCCGT1400.057.51283352
AGATCTC1400.057.51283340
CGTATCA1400.057.51283356
CGCCGTA1400.057.51283353
TCGGTGG1600.056.86837845
Produced by FastQC (version 0.11.5)