FastQCFastQC Report
Wed 25 May 2016
SRR1780805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147256
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2395616.268267506926712No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG46673.169310588363123No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT33652.2851360895311568No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC16861.1449448579344814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13000.8828163198783071No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT9710.6593958820014125No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT8290.5629651762916281No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT6640.45091541261476614No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC6010.40813277557450967No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT5040.3422610963220514Illumina PCR Primer Index 11 (95% over 22bp)
TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG4240.287933938175694No Hit
CAAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3650.24786765904275548No Hit
CATAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3470.2356440484598251No Hit
CACTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2140.14532514804150595No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG2070.1405715217036997No Hit
CATATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT1980.13445971641223448No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT1890.12834791112076926No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT1800.12223610582930407No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG1550.10525886890856738No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAGCTCCATCTCGTATGCCGT1500.10186342152442006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAT150.002218650270.0190253
TCACGAA150.002221641869.9952538
AACGTAT150.002221641869.9952539
ATCAACG207.9083766E-569.9952510
GCCGACA401.3460522E-1069.9952546
AACTACA150.002221641869.9952546
GACTCTC252.8298437E-669.9952529
CCTTGAA150.002221641869.9952556
AGGGGGG35750.065.41637470
ACGACGA501.4551915E-1162.9957227
CCGACAT453.8198777E-1062.21799547
CGACGAT401.0591975E-861.24584228
AAATCGT352.9594412E-759.99592637
CTTTATA700.059.9959262
AATCGTA352.9594412E-759.99592638
CAAATCG308.347373E-658.32937236
CTGATTG553.45608E-1157.2882954
ACGCACC553.45608E-1157.26883730
AACTCGT553.45608E-1157.26883737
TGACGTC1850.056.75290345
Produced by FastQC (version 0.11.5)