FastQCFastQC Report
Wed 25 May 2016
SRR1780804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329334
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4873214.797136038186157No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG6880.2089064597035229No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT200.006946819452.50159569
ACGTATA200.006946819452.50159570
CGTCATT200.006946819452.50159514
GCGTAAG200.006946819452.50159520
CGCGTCT305.87238E-446.6680877
TCGCCGT1450.041.02949552
TAAGCGG350.00125427740.0012175
TGGTCGC1400.040.00121749
GCCGTAT1400.039.99514454
ATCTCGG1700.039.11883542
AGATCTC1750.038.00115240
GATCTCG1800.036.94556841
CTCGGTG1650.036.061744
TCGGTGG1650.036.061745
GTCGCCG1750.035.9956351
CGTATCA1700.035.00106456
CGTATAA400.00241657535.00106433
GGAACGT501.707534E-435.00106437
CGAATTG501.707534E-435.00106448
CCGAATT501.707534E-435.00106447
Produced by FastQC (version 0.11.5)