FastQCFastQC Report
Wed 25 May 2016
SRR1780804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329334
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT251437.634498715589645No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG47601.4453412037627453No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT32850.9974676164623149No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC18640.5659907571037306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13860.42084935050738764No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT9600.2914973856328226No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT7920.24048534314707865No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC5080.15425069989736861No Hit
CAAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4500.13663939951538562No Hit
TCATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG4370.13269203908494112No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT3990.1211536009036419TruSeq Adapter, Index 9 (95% over 21bp)
CATAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3680.111740664492582No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC150.002223950369.9963526
CGTCCTG150.002223950369.9963534
AGGGGGG40400.064.8084570
CGAATGC750.060.6727241
AAGCCGT1250.058.79694444
GACGACT252.3891382E-455.9970946
ATCGTAT1450.055.52278539
ATATCGT1100.054.09630237
AGAGACG653.6379788E-1253.8433523
GCCGTCT51750.053.83294346
CCGTCTT51050.053.8169847
TCTTCTG50500.053.77937750
CGTCTTC51700.053.68192348
GATCTCG54100.053.50811436
AGATCTC53700.053.450435
GTCTTCT51150.053.36965649
CTTCTGC51350.053.36625751
ATCTCGT53750.053.33555637
TCTCGTA54400.053.27737838
TCGTATG55200.053.2027740