FastQCFastQC Report
Wed 25 May 2016
SRR1780803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751466
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG521526.940034545807794No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG8700.1157737010057674No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGT150.00222454570.00046535
CATCGTT404.5931E-543.7502932
TCGCCGT1800.042.7809152
CTCGGTG2050.039.27116844
TCGGTGG2050.039.27116845
GCCGTAT1800.038.89173554
GTCGCCG2200.036.59115651
TGGTCGC2200.036.59115649
CGGTGGT2400.032.08568246
TTCGTAG552.992359E-431.81839635
GATCTCG2450.031.42878241
GTATACG450.00430597831.11338844
TTAAACG450.00430597831.11338814
CGCCGTA2250.031.11338853
TATACGG450.00430597831.11338845
CTATCGG450.004307380431.11131942
TCTCGGT2400.030.62724143
ATCTCGG2500.029.40019642
TCGTTGT604.9824757E-429.1668634
GGGTCTA853.954343E-628.8256381
Produced by FastQC (version 0.11.5)