FastQCFastQC Report
Wed 25 May 2016
SRR1780803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751466
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT241453.2130528859589123No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG43680.5812638229806804No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT27940.37180657541392426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15250.20293665980896008No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC15000.1996098293202886No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT12200.16234932784716807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG34450.061.5697770
GCCGTCA7250.056.48109446
TATGCCG47650.053.4681143
CCGTCAT8250.053.45297247
AAGCCGT1900.053.4194844
CGTATGC44650.053.22351541
TCGTATG46900.053.05813640
GCCGTCT37600.053.0569546
CCGTCTT37600.052.77770247
TGCCGTA4250.052.7043345
CGTCTTA4850.052.67535448
GTATGCC48450.052.5852542
TGCCGTC44150.052.55790745
ATGCCGT48400.052.4261444
CGTCTTC34250.052.41832748
CTCGTAT47800.052.355539
TCTCGTA49800.051.86939238
ATCTCGT50150.051.57718737
CGTCATC7100.051.26268448
GTCTTCT32150.050.61722649