FastQCFastQC Report
Wed 25 May 2016
SRR1780802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60946
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4436972.80051192859253No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG6871.127227381616513No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1100.18048764480031504No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850.13946772552751616No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850.13946772552751616No Hit
TCTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGT840.1378269287566042No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.12962294490204443No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.12141896104748465No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.11977816427657272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG640.1050109933383651No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610.10008860302562923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGAT150.002211674170.0246566
CGTAGCA150.002211674170.0246556
CTGCCGG252.8029026E-670.0246518
TTGTGCG207.8548146E-570.024652
GTTGTGC150.002211674170.024651
GTCGGCG150.002211674170.0246551
CCGGCGA207.8548146E-570.0246521
GGTCGGC150.002211674170.0246550
GGATGCT150.00221889969.9671670
TGTGCGG150.00221889969.967163
TGCCGGC308.268233E-658.35387419
GCGCTGC308.268233E-658.35387415
GCCGGCG509.531504E-1056.0197220
GGCCGGC200.006910966752.5184919
TTATACG200.006910966752.518492
CGGTGGC200.006910966752.5184916
TGGCGCT407.348917E-752.5184913
TAGCATA200.006933477752.4753729
AGGTGGC200.006933477752.47537210
CGGCGAG200.006933477752.47537222
Produced by FastQC (version 0.11.5)