FastQCFastQC Report
Wed 25 May 2016
SRR1780802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60946
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2328038.197748826830306No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG45297.4311685754602435No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT32185.28008400879467RNA PCR Primer, Index 19 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC12852.108423850621862No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12362.028024808847176No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT8101.3290453844386834RNA PCR Primer, Index 19 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT6291.0320611689036197No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC5190.8515735241033046No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT4050.6645226922193417No Hit
TCATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3810.6251435697174548No Hit
CAAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3520.5775604633610081No Hit
CATAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3400.5578709021100646RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT2290.37574246053883764No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC2090.34292652512059857No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTTAAAATCTCGTATGCCGT1640.2690906704295606No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT1620.2658090768877367No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC1540.252682702720441No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG1540.252682702720441No Hit
CTTATACACATCTCCGCGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1500.24611951563679324No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC1370.22478915761493784RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACAAATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1310.2149443769894661No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC1220.20017720605125847No Hit
CTAAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1200.1968956125094346RNA PCR Primer, Index 19 (95% over 21bp)
CTTATAAACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1170.19197322219669874RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT1150.1886916286548748RNA PCR Primer, Index 19 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG1100.18048764480031504No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT1090.17884684802940307No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAGT1080.17720605125849112No Hit
CTTATACACATCTCCGAGACCACGAGACGCGTTAAAATCTCGTATGCCGT940.15423489646572375RNA PCR Primer, Index 19 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC830.13618613198569224RNA PCR Primer, Index 19 (95% over 22bp)
CAAAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT830.13618613198569224No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAACTCTCGTATGCCGT820.1345453352147803No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC790.12962294490204443No Hit
TCTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG780.12798214813113248No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT710.11649657073474878RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAACATCTCGTATGCCGT710.11649657073474878No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATATCGTATGCCGT710.11649657073474878No Hit
CTTATACACATATCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT690.11321497719292489No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAG680.11157418042201292No Hit
CTTATACACATCTCCGAGCACACGAGACGCGTTAAAATCTCGTATGCCGT670.10993338365110099RNA PCR Primer, Index 19 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG660.10829258688018903No Hit
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT660.10829258688018903No Hit
CATATACAAATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT660.10829258688018903RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTTAAATCTCGTATGCCGT640.1050109933383651No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT640.1050109933383651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATA150.002203543770.0896451
CTAAAAC309.991527E-870.0896451
TCTTTAT500.070.089641
CATATAA353.597961E-970.089641
CATAAAC850.070.089641
CTACAGC150.00221074870.0320551
CGCGACG150.00221074870.0320523
AATCAGC150.00221074870.0320552
CAACACC150.00221074870.032059
ACATCTG301.0048643E-770.0320550
TCTACAG150.00221074870.0320550
TTAAGCA150.00221074870.0320552
CGACGCG150.00221074870.0320525
CTTAAGC150.00221074870.0320551
TCATCTC150.00221074870.032058
ACGCGAC150.00221074870.0320522
ACAGCTT150.00221074870.0320553
AATACAA252.8011455E-670.032043
ACGCGAA353.621608E-970.0320427
ATCTGCA207.850709E-570.0320452
Produced by FastQC (version 0.11.5)