FastQCFastQC Report
Wed 25 May 2016
SRR1780801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241580
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6553627.12807351601954No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG11080.4586472390098518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG150.002222536670.0018637
CGGTATC150.002222536670.0018655
TACGCGT150.002224362769.987375
ACGCGTC150.002224362769.987376
TATTTCG200.006944691852.501437
CGAATCA200.006944691852.501456
CCGGTTC200.006944691852.501417
CAATGCG200.006944691852.501416
CACCGTA200.006944691852.501465
CCGTGTG200.006950380752.49053228
TGCACGT407.468825E-752.4905325
TCGCCGT1650.050.91044652
CGCCGTA1700.049.41308253
TAGATCT2150.048.83851239
CGTATCA1650.048.78917756
CCGTATC1650.048.78917755
TCTCGGT2100.048.34462743
GATCTCG2150.047.22033741
GTGGTCG2450.047.14411548
CGGTGGT2400.046.6775746
Produced by FastQC (version 0.11.5)