FastQCFastQC Report
Wed 25 May 2016
SRR1780801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241580
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3284613.5963241990231No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG60782.515936749730938No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT45451.881364351353589No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20280.8394734663465517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC16460.6813477936915308No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT11660.482655848994122No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT9780.4048348373209703No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC7650.31666528686149514No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT7050.2918287937743191No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT6090.2520904048348373No Hit
TCATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG5760.23843033363689048No Hit
CAAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5610.23222121036509646No Hit
CATAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5450.22559814554184948No Hit
CTTATACACATCTCCGCGCCCACGAGACCCAAATAAATCTCGTATGCCGT2640.10928056958357481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG252.833005E-670.0049723
TATGCGT150.00222397169.9904844
CGACTAA150.00222397169.9904848
CTCGTAA950.066.3067639
AGGGGGG50000.066.08469470
TCGTAAG1100.063.6277140
TCGAAAG401.0635631E-861.2416740
TCGAATG1450.060.3366240
ACTCGTA1050.059.9918438
AATGACG352.9688636E-759.99183743
TTTATAC1400.059.9918373
GTAATCT308.3668965E-658.32539749
CGTAATC308.3668965E-658.32539748
CGTCAAC308.3668965E-658.32539748
AAGCCGT1450.057.92315344
AACTCGT1100.057.2649437
TAAACAC4500.056.770053
CTTTATA1550.056.4439352
ATATCGA252.3888501E-455.99238237
CGTAAGC1250.055.99238241
Produced by FastQC (version 0.11.5)