FastQCFastQC Report
Wed 25 May 2016
SRR1780800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73647
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5225370.95061577525222No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG8511.1555121050416175No Hit
TCTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGT1150.15615028446508342No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.142571998859424No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG930.1262780561326327No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG870.11813108476923703No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850.11541542764810515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCGC150.002213284870.0265112
TTGGGCG150.002213284870.026512
GCGCTGC150.002213284870.0265115
TGGCGCT150.002213284870.0265113
GTGCGCG207.8646925E-570.026514
CCGTAAC150.00221926469.9789456
GCCGTAA150.00221926469.9789455
CTTGGGC252.3718596E-456.021211
TCTGGCG200.00691595752.51988611
GCGCGTC200.00691595752.5198866
CTGCAGG200.00691595752.5198861
GACGCCG200.00693458752.4842152
CGTAACA200.00693458752.4842157
CGGCTGG200.00693458752.484219
CGTCTGG200.00693458752.484219
CCGTATC1400.044.9864655
GCCGTAT1400.044.9864654
GTATCAT1500.044.31999657
TATCATT1500.044.31999658
GGCGCTG404.5426204E-543.7665714