FastQCFastQC Report
Wed 25 May 2016
SRR1780800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73647
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2671436.2730321669586No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG48996.652002118212555No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT30724.171249338058577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15842.1508004399364533No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC14311.9430526701698643No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT8001.0862628484527543No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT6140.8337067361874889No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC5680.7712466224014556No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT3910.5309109671812836TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT3850.522763995817888No Hit
TCATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3660.4969652531671351No Hit
CAAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3030.41142205385148073No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT2870.38969679688242564No Hit
CATAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2860.38833896832185966No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT1910.2593452550680951No Hit
CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1820.24712479802300164No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT1750.23761999809904003No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1750.23761999809904003No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1640.22268388393281466No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1630.2213260553722487No Hit
CACTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1490.2023164555243255No Hit
CTTATACAAATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1340.18194902711583635No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1300.17651771287357257No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT1290.17515988431300664No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT1260.1710863986313088No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT1180.16022377014678127No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1150.15615028446508342No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1150.15615028446508342No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCG1050.142571998859424No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT1000.1357828560565943No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA980.1330671989354624No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT970.13170937037489647No Hit
CTTATAAACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT960.13035154181433053No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT960.13035154181433053No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT890.12084674189036892No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT870.11813108476923703No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT860.11677325620867109No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT820.11134194196640733No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACG780.10591062772414356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACAGG252.8105678E-670.01766
CACGTAA207.8699886E-570.01739
TCAGAAT207.8699886E-570.01753
GCTTGTA207.8699886E-570.01756
ACCTTGA207.8699886E-570.01727
ATGCAGA252.8105678E-670.01744
TCGTAAT207.8699886E-570.01740
AATATGC207.8699886E-570.01751
CAGAATG207.8699886E-570.01754
ATCGTAA353.6434358E-970.01739
AGCCGTA207.8699886E-570.01745
CTTGAGT252.8105678E-670.01729
ACAGGGG252.8105678E-670.01768
GCCGAAT252.8105678E-670.01746
AAGCCGA252.8105678E-670.01744
CTTCTGA353.6434358E-970.01751
GCCCTCA150.002214478670.0169946
AATCAGT150.002214478670.0169944
TGCCGAA301.00930265E-770.0169945
TTCTGAT301.00930265E-770.0169952