FastQCFastQC Report
Wed 25 May 2016
SRR1780799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281759
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6930824.598326938979767No Hit
CTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTG11510.40850514091830253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG252.8369213E-669.9937922
TACGCGT301.02210834E-769.993795
ACGCGTC308.368152E-658.328166
TTCCACG252.3868826E-456.0049734
ATACGCG252.3889772E-455.9950334
GTATACC200.00693930552.513981
GTACCAT200.00694417952.5046651
TTCAGTA200.00694417952.5046647
CAACGGT200.00694417952.5046650
AACGGTC200.00694417952.5046651
CCAGTTA200.00694417952.5046614
CGTCATT200.00694417952.5046648
CCGCACC200.00694417952.5046634
TCACGTC200.00694417952.5046645
CTTCGGT200.00694905652.49534660
AGTCCGC200.00694905652.49534619
TCTTCGG200.00694905652.49534659
CTGGTAA200.00694905652.49534654
CCGTAGT200.00694905652.49534625
CCCCGGT200.00694905652.4953467
Produced by FastQC (version 0.11.5)